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Methodological Approaches to Investigate the Effects of Histamine Receptor Targeting Compounds in Preclinical Models of Breast Cancer

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Histamine Receptors as Drug Targets

Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

Abstract

Numerous studies demonstrated the pivotal role of histamine in breast cancer development and progression. This chapter aims to describe different preclinical breast cancer models, including in vitro soft agar clonogenic assay and in vivo chemically induced breast tumors in rats, human triple negative breast cancer (TNBC) xenograft, and murine TNBC syngeneic graft in mice. All these models could be useful and complementary to assess the role of histamine receptor ligands in breast cancer, which could originate advances in novel therapeutics to treat this disease. This chapter also describes the gold standard radiometric techniques to evaluate the activity of histamine metabolizing enzymes in breast cancer specimens.

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Authors and Affiliations

  1. Laboratory of Radioisotopes, School of Pharmacy and Biochemistry, University of Buenos Aires, Junín 956, PB 1113, Buenos Aires, Argentina

    Diego J. Martinel Lamas, Graciela P. Cricco, Gabriela A. Martin, Graciela A. Cremaschi, Elena S. Rivera & Vanina A. Medina

  2. Laboratory of Tumor Biology and Inflammation, Institute for Biomedical Research (BIOMED), School of Medical Sciences, Pontifical Catholic University of Argentina (UCA), and the National Scientific and Technical Research Council (CONICET), Buenos Aires, Argentina

    Diego J. Martinel Lamas, Melisa B. Nicoud & Vanina A. Medina

  3. Neuroimmunomodulation and Molecular Oncology Division, Institute for Biomedical Research (BIOMED), School of Medical Sciences, Pontifical Catholic University of Argentina (UCA), and the National Scientific and Technical Research Council (CONICET), Buenos Aires, Argentina

    Helena Sterle & Graciela A. Cremaschi

  4. National Scientific and Technical Research Council (CONICET), Buenos Aires, Argentina

    Gabriela A. Martin

  5. Molecular Biology Laboratory, Department of Visceral, Transplant and Thoracic Surgery, Medical University Innsbruck, Innsbruck, Austria

    Hubert G. Schwelberger

Authors
  1. Diego J. Martinel Lamas
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  2. Melisa B. Nicoud
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  4. Graciela P. Cricco
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  5. Gabriela A. Martin
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  6. Graciela A. Cremaschi
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  7. Hubert G. Schwelberger
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  8. Elena S. Rivera
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  9. Vanina A. Medina
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Correspondence to Vanina A. Medina .

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Editors and Affiliations

  1. Department of Pharmacology Medical School, National and Kapodistrian University of Athens, Athens, Greece

    Ekaterini Tiligada

  2. Centre for Experimental Medicine, School of Medicine, Dentistry and Biomedical Sciences, Queen’s University Belfast, Belfast, Northern Ireland, United Kingdom

    Madeleine Ennis

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Lamas, D.J.M. et al. (2017). Methodological Approaches to Investigate the Effects of Histamine Receptor Targeting Compounds in Preclinical Models of Breast Cancer. In: Tiligada, E., Ennis, M. (eds) Histamine Receptors as Drug Targets. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6843-5_12

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  • DOI: https://doi.org/10.1007/978-1-4939-6843-5_12

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