Abstract
The volumetric bar-chart chip (V-Chip) is a microfluidics-based, point-of-care (POC) device for the multiplexed and quantitative measurement of biomarkers. Volumetric readouts, based on the measurement of oxygen generated by a reaction between catalase and hydrogen peroxide, allow instant visual quantitation of target biomarkers and provide visualized bar charts without any assistance from instruments and without the need for data processing or graphics plotting. V-Chip shows potential capabilities in POC and personalized diagnostics; for instance, it can be utilized for making high-throughput, multiplexed, and quantitative measurements. Further, this system is highly portable and can be performed at low cost. The development of the V-Chip thus marks a POC milestone and opens up the possibility of instrument-free personalized diagnostics. Here, we describe the protocols for the fabrication of V-Chip and the use of silica nanoparticles as the probe carrier for the V-Chip-based enzyme-linked immunosorbent assay (ELISA) for the detection of biomarkers.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Song Y, Zhang Y, Bernard PE, Reuben JM, Ueno NT, Arlinghaus RB, Zu Y, Qin L (2012) Multiplexed volumetric bar-chart chip for point-of-care diagnostics. Nat Commun 3:12831–12839
Duan B, Hockaday LA, Kang KH, Butcher JT (2013) 3D bioprinting of heterogeneous aortic valve conduits with alginate/gelatin hydrogels. J Biomed Mater Res A 101:1255–1264
Gervais L, de Rooij N, Delamarche E (2011) Microfluidic chips for point-of-care immunodiagnostics. Adv Mater 23:H151–H176
Chin CD, Laksanasopin T, Cheung YK, Steinmiller D, Linder V, Parsa H, Wang J, Moore H, Rouse R, Umviligihozo G, Karita E, Mwambarangwe L, Braunstein SL, van de Wijgert J, Sahabo R, Justman JE, El-Sadr W, Sia SK (2011) Microfluidics-based diagnostics of infectious diseases in the developing world. Nat Med 17:1015–1019
Fan R, Vermesh O, Srivastava A, Yen BK, Qin L, Ahmad H, Kwong GA, Liu CC, Gould J, Hood L, Heath JR (2008) Integrated barcode chips for rapid, multiplexed analysis of proteins in microliter quantities of blood. Nat Biotechnol 26:1373–1378
Stern E, Vacic A, Rajan NK, Criscione JM, Park J, Ilic BR, Mooney DJ, Reed MA, Fahmy TM (2010) Label-free biomarker detection from whole blood. Nat Nanotechnol 5:138–142
Xiang Y, Lu Y (2011) Using personal glucose meters and functional DNA sensors to quantify a variety of analytical targets. Nat Chem 3:697–703
Song YJ, Huang YY, Liu XW, Zhang XJ, Ferrari M, Qin LD (2014) Point-of-care technologies for molecular diagnostics using a drop of blood. Trends Biotechnol 32:132–139
Addae-Mensah KA, Cheung YK, Fekete V, Rendely MS, Sia SK (2010) Actuation of elastomeric microvalves in point-of-care settings using handheld, battery-powered instrumentation. Lab Chip 10:1618–1622
Qin LD, Vermesh O, Shi QH, Heath JR (2009) Self-powered microfluidic chips for multiplexed protein assays from whole blood. Lab Chip 9:2016–2020
Du WB, Li L, Nichols KP, Ismagilov RF (2009) SlipChip. Lab Chip 9:2286–2292
Belder D (2010) Screening in one sweep using the slipchip. Angew Chem Int Ed Engl 49:6484–6486
Li L, Du WB, Ismagilov RF (2010) User-loaded SlipChip for equipment-free multiplexed nanoliter-scale experiments. J Am Chem Soc 132:106–111
Shen F, Davydova EK, Du WB, Kreutz JE, Piepenburg O, Ismagilov RF (2011) Digital isothermal quantification of nucleic acids via simultaneous chemical initiation of recombinase polymerase amplification reactions on SlipChip. Anal Chem 83:3533–3540
Shen F, Du WB, Davydova EK, Karymov MA, Pandey J, Ismagilov RF (2010) Nanoliter multiplex PCR arrays on a SlipChip. Anal Chem 82:4606–4612
Shen F, Sun B, Kreutz JE, Davydova EK, Du WB, Reddy PL, Joseph LJ, Ismagilov RF (2011) Multiplexed quantification of nucleic acids with large dynamic range using multivolume digital RT-PCR on a rotational SlipChip tested with HIV and hepatitis C viral load. J Am Chem Soc 133:17705–17712
Li Y, Xuan J, Song Y, Qi W, He B, Wang P, Qin L (2016) Nanoporous glass integrated in volumetric bar-chart chip for point-of-care diagnostics of non-small cell lung cancer. ACS Nano 10:1640–1647
Li Y, Xuan J, Xia T, Han X, Song Y, Cao Z, Jiang X, Guo Y, Wang P, Qin L (2015) Competitive volumetric bar-chart chip with real-time internal control for point-of-care diagnostics. Anal Chem 87:3771–3777
Li Y, Xuan J, Song Y, Wang P, Qin L (2015) A microfluidic platform with digital readout and ultra-low detection limit for quantitative point-of-care diagnostics. Lab Chip 15:3300–3306
George P (1947) Reaction between catalase and hydrogen peroxide. Nature 160:41–43
Song Y, Wang Y, Qin L (2013) A multistage volumetric bar chart chip for visualized quantification of DNA. J Am Chem Soc 135:16785–16788
Song Y, Xia X, Wu X, Wang P, Qin L (2014) Integration of platinum nanoparticles with a volumetric bar-chart chip for biomarker assays. Angew Chem Int Ed Engl 53:12451–12455
Eichelsdoerfer DJ, Liao X, Cabezas MD, Morris W, Radha B, Brown KA, Giam LR, Braunschweig AB, Mirkin CA (2013) Large-area molecular patterning with polymer pen lithography. Nat Protoc 8:2548–2560
Heyries KA, Tropini C, VanInsberghe M, Doolin C, Petriv OI, Singhal A, Leung K, Hughesman CB, Hansen CL (2011) Megapixel digital PCR. Nat Methods 8:649–651
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Song, Y., Li, Y., Qin, L. (2017). Volumetric Bar-Chart Chips for Biosensing. In: Petrosko, S., Day, E. (eds) Biomedical Nanotechnology. Methods in Molecular Biology, vol 1570. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6840-4_7
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6840-4_7
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6838-1
Online ISBN: 978-1-4939-6840-4
eBook Packages: Springer Protocols