Abstract
Gold nanomaterials with light-responsive properties can be exploited as light-triggered delivery vehicles to enhance the therapeutic efficacy of anticancer drugs. Additionally, different wavelengths of light can be utilized to achieve the combined effects of light-triggered release of therapeutics and light-induced localized heating, which results in improved anticancer efficacy. Herein, we describe methods to develop gold nanorod (AuNR) complexes that provide drug delivery or photothermal therapy when activated by ultraviolet (UV) or near-infrared (NIR) wavelengths of light, respectively. The surface functionalization of AuNRs with three key components is presented. The first component, cyclodextrin, serves to encapsulate drugs of interest. The second component, dextran-phenyl-azo-benzoic acid (DexAzo), serves as a capping agent that undergoes a conformational change upon UV light activation to expose the drugs for release. The third component is a folic acid-based targeting ligand that provides efficient delivery of the AuNR complexes to cancer cells. The dual wavelength activation of these drug-loaded AuNR complexes, which enables one to achieve highly efficient anticancer therapy through the combined effects of UV-triggered drug release and NIR-induced hyperthermia, is also described.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Mura S, Nicolas J, Couvreur P (2013) Stimuli-responsive nanocarriers for drug delivery. Nat Mater 12:991–1003
Davis ME, Chen Z, Shin DM (2008) Nanoparticle therapeutics: an emerging treatment modality for cancer. Nat Rev Drug Discov 7:771–782
Brannon-Peppas L, Blanchette JO (2012) Nanoparticle and targeted systems for cancer therapy. Adv Drug Deliv Rev 64:206–212
Pacardo DB, Ligler FS, Gu Z (2015) Programmable nanomedicine: synergistic and sequential drug delivery systems. Nanoscale 7:3381–3391
Wang Y, Shim MS, Levinson NS, Sung H-W, Xia Y (2014) Stimuli-responsive materials for controlled release of theranostic agents. Adv Funct Mater 24:4206–4220
Cheng R, Meng F, Deng C, Klok H-A, Zhong Z (2013) Dual and multi-stimuli responsive polymeric nanoparticles for programmed site-specific drug delivery. Biomaterials 34:3647–3657
Hauck TS, Jennings TL, Yatsenko T, Kumadras JC, Chan WCW (2008) Enhancing the toxicity of cancer chemotherapeutics with gold nanorod hyperthermia. Adv Mater 20:3832–3838
Wang Z, Zong S, Yang J, Li J, Cui Y (2011) Dual-mode probe based on mesoporous silica coated gold nanorods for targeting cancer cells. Biosens Bioelectron 26:2883–2889
Terentyuk G, Panfilova E, Khanadeev V, Chumakov D, Genina E, Bashkatov A, Tuchin V, Bucharskaya A, Maslyakova G, Khlebtsov N, Khlebtsov B (2014) Gold nanorods with a hematoporphyrin-loaded silica shell for dual-modality photodynamic and photothermal treatment of tumors in vivo. Nano Res 7:325–337
Pacardo DB, Neupane B, Rikard SM, Lu Y, Mo R, Mishra SR, Tracy JB, Wang G, Ligler FS, Gu Z (2015) A dual wavelength-activatable gold nanorod complex for synergistic cancer treatment. Nanoscale 7:12096–12103
Merrill NA, Sethi M, Knecht MR (2013) Structural and equilibrium effects of the surface passivant on the stability of au nanorods. ACS Appl Mater Interfaces 5:7906–7914
Sau TK, Murphy CJ (2004) Seeded high yield synthesis of short au nanorods in aqueous solution. Langmuir 20:6414–6420
Wondraczek H, Heinze T (2008) Efficient synthesis and characterization of new photoactive dextran esters showing nanosphere formation. Macromol Biosci 8:606–614
Pacardo DB, Neupane B, Wang G, Gu Z, Walker GM, Ligler FS (2015) A temperature microsensor for measuring laser-induced heating in gold nanorods. Anal Bioanal Chem 407:719–725
Smith DK, Miller NR, Korgel BA (2009) Iodide in CTAB prevents gold nanorod formation. Langmuir 25:9518–9524
Alkilany AM, Nagaria PK, Hexel CR, Shaw TJ, Murphy CJ, Wyatt MD (2009) Cellular uptake and cytotoxicity of gold nanorods: molecular origin of cytotoxicity and surface effects. Small 5:701–708
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Pacardo, D.B., Ligler, F.S., Gu, Z. (2017). Dual Wavelength-Triggered Gold Nanorods for Anticancer Treatment. In: Petrosko, S., Day, E. (eds) Biomedical Nanotechnology. Methods in Molecular Biology, vol 1570. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6840-4_13
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6840-4_13
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6838-1
Online ISBN: 978-1-4939-6840-4
eBook Packages: Springer Protocols