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Assessment of Cytokinin-Induced Immunity Through Quantification of Hyaloperonospora arabidopsidis Infection in Arabidopsis thaliana

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Auxins and Cytokinins in Plant Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1569))

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Abstract

Cytokinins have been shown to regulate plant immunity. Application of high levels of cytokinin to plants leads to decreased susceptibility to pathogens. In this chapter, we describe a fast and accurate protocol for assessment of cytokinin-induced immunity in Arabidopsis plants against an oomycete plant pathogen.

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References

  1. Choi J, Huh SU, Kojima M, Sakakibara H, Paek K-H, Hwang I (2010) The cytokinin-activated transcription factor ARR2 promotes plant immunity via TGA3/NPR1-dependent salicylic acid signaling in Arabidopsis. Dev Cell 19:284–295

    Article  CAS  PubMed  Google Scholar 

  2. Argueso CT, Ferreira FJ, Epple P, To JPC, Hutchison CE, Schaller GE, Dangle JL, Kieber JJ (2012) Two-component elements mediate interactions between cytokinin and salicylic acid in plant immunity. PLoS Genet 8:e1002448

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Naseem M, Philippi N, Hussain A, Wangorsch G, Ahmed N, Dandekar T (2012) Integrated systems view on networking by hormones in Arabidopsis immunity reveals multiple crosstalk for cytokinin. Plant Cell 24:1793–1814

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Grosskinsky DK, Naseem M, Abdelmohsen UR, Plickert N, Engelke T, Zeier J, Novak O, Strnad M, Pfeifhofer H, Van der Graaf E, Simon U, Roitsch T (2011) Cytokinins mediate resistance against Pseudomonas syringae in tobacco through increased antimicrobial phytoalexin synthesis independent of salicylic acid signaling. Plant Physiol 157:815–830

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Jiang C-J, Shimono M, Sugano S, Kojima M, Liu X, Inoue H, Sakakibara H, Takatsuji H (2013) Cytokinins act synergistically with salicylic acid to activate defense gene expression in rice. Mol Plant Microbe Interact 26:287–296

    Article  CAS  PubMed  Google Scholar 

  6. Conrath U, Beckers GJM, Langenbach CJG, Jaskiewicz MR (2015) Priming for enhanced defense. Annu Rev Phytopathol 53:97–119

    Article  CAS  PubMed  Google Scholar 

  7. Coates ME, Beynon JL (2010) Hyaloperonospora arabidopsidis as a pathogen model. Annu Rev Phytopathol 48:329–345

    Article  CAS  PubMed  Google Scholar 

  8. Holub EB, Beynon LJ, Crute IR (1994) Phenotypic and genotypic characterization of interactions between isolates of Peronospora parasitica and accessions of Arabidopsis thaliana. Mol Plant Microbe Interact 7:223–239

    Article  CAS  Google Scholar 

  9. Feys BJ, Moisan LJ, Newman MA, Parker JE (2001) Direct interaction between the Arabidopsis disease resistance signaling proteins, EDS1 and PAD4. EMBO J 20:5400–5411

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Tome D, Steinbrenner J, Beynon JL (2014) A growth quantification assay for Hyaloperonospora arabidopsidis isolates in Arabidopsis thaliana. Methods Mol Biol 1127:145–156

    Article  PubMed  Google Scholar 

  11. McDowell JM, Hoff T, Anderson RG, Deegan D (2011) Propagation, storage, and assays with Hyaloperonospora arabidopsidis: a model oomycete pathogen of Arabidopsis. Methods Mol Biol 712:137–151

    Article  CAS  PubMed  Google Scholar 

  12. Anderson RG, McDowell JM (2015) A PCR assay for the quantification of growth of the oomycete pathogen Hyaloperonospora arabidopsidis in Arabidopsis thaliana. Mol Plant Pathol 16:893–898

    Article  CAS  PubMed  Google Scholar 

  13. Zheng X-Y, Zhou M, Yoo H, Pruneda-Paz JL, Spivey NW, Kay SA, Dong X (2015) Spatial and temporal regulation of biosynthesis of the plant immune signal salicylic acid. Proc Natl Acad Sci U S A 112:9166–9173

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  14. Wang W, Barnaby JY, Tada Y, Li H, Toer M, Caldelari D, Lee D-U, Fu X-D, Dong X (2011) Timing of plant immune responses by a central circadian regulator. Nature 470:110–U126

    Article  CAS  PubMed  Google Scholar 

  15. Zhang C, Xie Q, Anderson RG, Ng G, Seitz NC, Peterson T, McClung CR, McDowell JM, Kong D, Kwak JM, Lu H (2013) Crosstalk between the circadian clock and innate immunity in Arabidopsis. PLoS Pathog 9:e1003370

    Google Scholar 

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Acknowledgments

We thank Phil Turk from the Franklin Graybill Statistical Laboratory at Colorado State University for the help with statistical analysis and Bettina Broeckling for comments on the manuscript.

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Correspondence to Cristiana T. Argueso .

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Watson, R.A., Argueso, C.T. (2017). Assessment of Cytokinin-Induced Immunity Through Quantification of Hyaloperonospora arabidopsidis Infection in Arabidopsis thaliana . In: Dandekar, T., Naseem, M. (eds) Auxins and Cytokinins in Plant Biology. Methods in Molecular Biology, vol 1569. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6831-2_9

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  • DOI: https://doi.org/10.1007/978-1-4939-6831-2_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6829-9

  • Online ISBN: 978-1-4939-6831-2

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