Abstract
Plant physiology, in particular, is governed by a repertoire of endogenous as well as environmental cues. Auxin and cytokinin constitute an indispensable phytohormonal system required for plant growth and development. Another pivotal aspect of plant physiological process that thoroughly affects various plant growth and developmental attributes is the signaling network, majorly comprising the canonical mitogen activated protein kinase (MAPK) cascade. Striking a fine balance between the phytohormonal and signaling components could be adopted as an intricate strategy by plants to counteract various stresses in question. Thus, a brief understanding of this multifaceted complex could be of use for delineating numerous plant physiological and developmental phenomena. Thus, the present section discusses the various MAPK related assays in context to auxin and cytokinin crosstalk. Briefly, this chapter outlines the discrete MAPK methods to better understand the fundamentals of MAPK signaling network in auxin and cytokinin treated rice seedlings. Further, various phenotypic, genomic as well as proteomic protocols are discussed for a better understanding of MAPK networks in the backdrop of auxin and cytokinin interplay.
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References
Muller B, Sheen J (2008) Cytokinin and auxin interaction in root stem-cell specification during early embryogenesis. Nature 453:1094–1097. doi:10.1038/nature06943, PMID: 18463635
Bishopp A, Benkova E, Helariutta Y (2011) Sending mixed messages: auxin-cytokinin crosstalk in roots. Curr Opin Plant Biol 14:10–16. doi:10.1016/j.pbi.2010.08.014, PMID: 20926335
Colcombet J, Hirt H (2008) Arabidopsis MAPKs: a complex signalling network involved in multiple biological processes. Biochem J 413:217–226. doi:10.1042/BJ20080625, PMID: 18570633
Taj G, Agarwal P, Grant M, Kumar A (2010) MAPK machinery in plants: recognition and response to different stresses through multiple signal transduction pathways. Plant Signal Behav 5:1370–1378, PMID: 20980831
Doczi R, Okresz L, Romero AE, Paccanaro A, Bogre L (2012) Exploring the evolutionary path of plant MAPK networks. Trends Plant Sci 17:518–525. doi:10.1016/j.tplants.2012.05.009, PMID: 22682803
Singh PS, Mohanta TK, Sinha AK (2015) Unraveling the intricate nexus of molecular mechanisms governing rice root development: OsMPK3/6 and auxin-cytokinin interplay. PLoS One 10(4):e0123620. doi:10.1371/journal.pone.0123620
Sinha AK, Jaggi M, Raghuram B, Tuteja N (2011) Mitogen-activated protein kinase signaling in plants under abiotic stress. Plant Signal Behav 6:196–203
Rodriguez MC, Petersen M, Mundy J (2010) Mitogen-activated protein kinase signaling in plants. Annu Rev Plant Biol 61:621–649. doi:10.1146/annurev-arplant-042809-112252, PMID: 20441529
Galkovskyi T, Mileyko Y, Bucksch A, Moore B, Symonova O, Topp CN et al (2012) GiA Roots: software for the high throughput analysis of plant root system architecture. BMC Plant Biol 12:116. doi:10.1186/1471-2229-12-116, PMID: 22834569
Raghuram B, Sheikh AH, Sinha AK (2014) Regulation of MAP kinase signaling cascade by microRNAs in Oryza sativa. Plant Signal Behav 9
Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)). Methods 25:402–408, PMID: 11846609
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram 882 quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Raina SK, Wankhede DP, Jaggi M, Singh P, Jalmi SK, Raghuram B et al (2012) CrMPK3, a mitogen activated protein kinase from Catharanthus roseus and its possible role in stress induced biosynthesis of monoterpenoid indole alkaloids. BMC Plant Biol 12:134. doi:10.1186/1471-2229-12-134, PMID: 22871174
Rao KP, Vani G, Kumar K, Wankhede DP, Misra M, Gupta M et al (2011) Arsenic stress activates MAP kinase in rice roots and leaves. Arch Biochem Biophys 506:73–82. doi:10.1016/j.abb.2010.11.006, PMID: 21081102
Sethi V, Raghuram B, Sinha AK, Chattopadhyay S (2014) A. mitogen-activated protein kinase cascade module, MKK3-MPK6 and MYC2, is involved in blue light-mediated seedling development in Arabidopsis. Plant Cell 26:3343–3357. doi:10.1105/tpc.114.128702, PMID: 25139007
Acknowledgments
The authors extend their gratitude to the Department of Biotechnology (DBT), Government of India and National Institute of Plant Genome Research (NIPGR), New Delhi, India for financial support. Council of Scientific and Industrial Research (CSIR), Government of India is duly acknowledged for providing fellowship to PS.
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Singh, P., Sinha, A.K. (2017). Interplay Between Auxin and Cytokinin and Its Impact on Mitogen Activated Protein Kinase (MAPK). In: Dandekar, T., Naseem, M. (eds) Auxins and Cytokinins in Plant Biology. Methods in Molecular Biology, vol 1569. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6831-2_7
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DOI: https://doi.org/10.1007/978-1-4939-6831-2_7
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