Abstract
Parallel determination of auxin and cytokinin levels within plant organs and tissues represents an invaluable tool for studies of their physiological effects and mutual interactions. Thanks to their different chemical structures, auxins, cytokinins and their metabolites are often determined separately, using specialized procedures of sample purification, extraction, and quantification. However, recent progress in the sensitivity of analytical methods of liquid chromatography coupled to mass spectrometry (LC-MS) allows parallel analysis of multiple compounds. Here we describe a method that is based on single step purification protocol followed by LC-MS separation and detection for parallel analysis of auxins, cytokinins and their metabolites in various plant tissues and cell cultures.
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Acknowledgment
This work was supported by MEYS CR, projects no. LD14120 (PD), LD15137 (KH), and LO1417 (JP).
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Dobrev, P.I., Hoyerová, K., Petrášek, J. (2017). Analytical Determination of Auxins and Cytokinins. In: Dandekar, T., Naseem, M. (eds) Auxins and Cytokinins in Plant Biology. Methods in Molecular Biology, vol 1569. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6831-2_2
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DOI: https://doi.org/10.1007/978-1-4939-6831-2_2
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6831-2
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