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Chapter 5 Slow Freezing of Human Sperm

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Cryopreservation of Mammalian Gametes and Embryos

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1568))

Abstract

Cryopreservation of human spermatozoa is a highly efficient procedure for managing male fertility, and much of its successful application seems to have a crucial impact on the reproductive outcome of assisted reproduction technologies. Here, we present, explain, and describe the slow freezing method for preserving human spermatozoa, which is currently the most commonly used freezing technique in most clinical andrology laboratories.

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Author information

Authors and Affiliations

  1. American Center for Reproductive Medicine, Cleveland Clinic, 10681 Carnegie Ave., X11, Cleveland, OH, 44195, USA

    Ashok Agarwal & Eva Tvrda

  2. Department of Animal Physiology, Slovak University of Agriculture, Tr. A. Hlinku 2, 94976, Nitra, Slovak Republic

    Eva Tvrda

Authors
  1. Ashok Agarwal
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  2. Eva Tvrda
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Corresponding author

Correspondence to Ashok Agarwal .

Editor information

Editors and Affiliations

  1. Reproductive Biology Associates, Atlanta, Georgia, USA

    Zsolt Peter Nagy

  2. ASTRA Fertility Group, Mississauga, Ontario, Canada

    Alex C. Varghese

  3. Cleveland Clinic Foundation, Cleveland, Ohio, USA

    Ashok Agarwal

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Agarwal, A., Tvrda, E. (2017). Chapter 5 Slow Freezing of Human Sperm. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) Cryopreservation of Mammalian Gametes and Embryos. Methods in Molecular Biology, vol 1568. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6828-2_5

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  • DOI: https://doi.org/10.1007/978-1-4939-6828-2_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6826-8

  • Online ISBN: 978-1-4939-6828-2

  • eBook Packages: Springer Protocols

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