Skip to main content

Appendix G: Vitrification of Blastocysts Using VitriBlastTM and ThermoBlastTM: Nidacon

  • Protocol
  • First Online:
Cryopreservation of Mammalian Gametes and Embryos

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1568))

  • 1574 Accesses

Abstract

This appendix describes the vitrification of blastocysts using VitriBlast™ (VBK) and ThermoBlast™ (TBK) from Nidacon, Mölndal, Sweden. The technique used and the reason for not including DMSO in the medium at the production stage, but including it separately in the kit, and the importance of collapsing the blastocyst prior to vitrification will be explained and described.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Lane M, Forest KT et al (1999) Live births following vitrification of hamster embryos using a novel containerless technique. Theriogenology 51:67

    Article  Google Scholar 

  2. Loutradi K, Kolibianakis E et al (2008) Cryopreservation of human embryos by vitrification or slow freezing: a systematic review and meta analysis. Fertil Steril 90(1):186–193

    Article  PubMed  Google Scholar 

  3. Mukaida T, Takahashi K (2007) Vitrification of blastocysts using the cryoloop technique. In: Tucker M, Liemberman J (eds) Vitrification in assisted reproduction, a user’s manual and trouble-shooting guide. Informa, UK, pp 219–237

    Chapter  Google Scholar 

  4. Kasai M, Niwa K et al (1980) Survival of mouse embryos frozen and thawed rapidly. J Reprod Fertil 59:51–56

    Article  CAS  PubMed  Google Scholar 

  5. Kasai M, Nowee K et al (1983) Protective effect of scrose on the survival of mouse and rat embryos stored at 0 °C. J Reprod Fertil 68:377–380

    Article  CAS  PubMed  Google Scholar 

  6. Kasai M, Edashige K (2007) Vitrification in animal reproduction: vitrification of embryos using conventional straws with an ethylene glycol-based solutions. In: Tucker M, Liemberman J (eds) Vitrification in assisted reproduction, a user’s manual and trouble-shooting guide. Informa, UK, pp 75–85

    Chapter  Google Scholar 

  7. Kartberg A-J, Hambiliki F et al (2008) Vitrification with DMSO protects embryo membrane integrity better than solutions without DMSO. RBM Online 17(3):378–384

    PubMed  Google Scholar 

  8. Duus A, Lundin K (2008), Vitrification of human blastocysts with either DMSO or PrOH as a cryoprotectant. In: Poster presented at the 24th annual meeting of the European society of human reproduction and embryology (ESHRE), Barcelona, 6–9 July 2008

    Google Scholar 

  9. Abedini A, Singha G et al (2006) Recovery and purification of highly aggregation-prone disulfide containing peptides: application to islet amyloid polypeptide. Anal Biochem 351:181–186

    Article  CAS  PubMed  Google Scholar 

  10. Pogorelyi, V. K. Barvinchenko V. N et al (1988) Solvation effects in human serum albumin radioulysis in the presence of dimethyl sulfoxide. V. K. Teoreticheskaya i Eksperimental’naya Khimiya 26 107–110. Translated 1990

    Google Scholar 

  11. Vanderzwalmen P, Bertin G et al (2002) Births after vitrification at morula and blastocyst stages: effect of artificial reduction of the blastocoelic cavity before vitrification. Hum Reprod 17(3):744–751

    Article  PubMed  Google Scholar 

  12. Mukaida T, Oka C et al (2006) Artificial shrinkage of blastocoeles using either a micro-needle or a laser pulse prior to the cooling steps of vitrification improves survival rate and pregnancy outcome of vitrified human blastocysts. Hum Reprod 21(12):3246–3252

    Article  CAS  PubMed  Google Scholar 

  13. Zhang X, Trokoudes K et al (2009) Vitrification of biopsied embryos at cleavage, morula and blastocyst stage. RBM Online 19(4):526–531

    PubMed  Google Scholar 

  14. Son W-Y, Yoon S-H et al (2003) Pregnancy outcome following transfer of human blastocysts vitrified on electron microscopy grids after induced collapse of the blastocoele. Hum Reprod 18(1):137–139

    Article  CAS  PubMed  Google Scholar 

  15. Hardarson T et al (2006) Vitrification and warming human blastocysts by use of a laser to artificially induce blastocyst collapse prior to vitrification. Acta Obstet Gynecol Scand 86:119–120

    Google Scholar 

  16. Wikland M, Hardarson T et al (2010) Obstetric outcomes after transfer of vitrified blastocysts. Hum Reprod 27(7):1699–1707

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Anna Niläng Laessker .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer Science+Business Media LLC

About this protocol

Cite this protocol

Laessker, A.N., Hardarsson, T., Forsberg, AS., Mukaida, T., Holmes, P.V. (2017). Appendix G: Vitrification of Blastocysts Using VitriBlastTM and ThermoBlastTM: Nidacon. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) Cryopreservation of Mammalian Gametes and Embryos. Methods in Molecular Biology, vol 1568. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6828-2_27

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-6828-2_27

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6826-8

  • Online ISBN: 978-1-4939-6828-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics