Abstract
This appendix describes the vitrification of blastocysts using VitriBlast™ (VBK) and ThermoBlast™ (TBK) from Nidacon, Mölndal, Sweden. The technique used and the reason for not including DMSO in the medium at the production stage, but including it separately in the kit, and the importance of collapsing the blastocyst prior to vitrification will be explained and described.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Lane M, Forest KT et al (1999) Live births following vitrification of hamster embryos using a novel containerless technique. Theriogenology 51:67
Loutradi K, Kolibianakis E et al (2008) Cryopreservation of human embryos by vitrification or slow freezing: a systematic review and meta analysis. Fertil Steril 90(1):186–193
Mukaida T, Takahashi K (2007) Vitrification of blastocysts using the cryoloop technique. In: Tucker M, Liemberman J (eds) Vitrification in assisted reproduction, a user’s manual and trouble-shooting guide. Informa, UK, pp 219–237
Kasai M, Niwa K et al (1980) Survival of mouse embryos frozen and thawed rapidly. J Reprod Fertil 59:51–56
Kasai M, Nowee K et al (1983) Protective effect of scrose on the survival of mouse and rat embryos stored at 0 °C. J Reprod Fertil 68:377–380
Kasai M, Edashige K (2007) Vitrification in animal reproduction: vitrification of embryos using conventional straws with an ethylene glycol-based solutions. In: Tucker M, Liemberman J (eds) Vitrification in assisted reproduction, a user’s manual and trouble-shooting guide. Informa, UK, pp 75–85
Kartberg A-J, Hambiliki F et al (2008) Vitrification with DMSO protects embryo membrane integrity better than solutions without DMSO. RBM Online 17(3):378–384
Duus A, Lundin K (2008), Vitrification of human blastocysts with either DMSO or PrOH as a cryoprotectant. In: Poster presented at the 24th annual meeting of the European society of human reproduction and embryology (ESHRE), Barcelona, 6–9 July 2008
Abedini A, Singha G et al (2006) Recovery and purification of highly aggregation-prone disulfide containing peptides: application to islet amyloid polypeptide. Anal Biochem 351:181–186
Pogorelyi, V. K. Barvinchenko V. N et al (1988) Solvation effects in human serum albumin radioulysis in the presence of dimethyl sulfoxide. V. K. Teoreticheskaya i Eksperimental’naya Khimiya 26 107–110. Translated 1990
Vanderzwalmen P, Bertin G et al (2002) Births after vitrification at morula and blastocyst stages: effect of artificial reduction of the blastocoelic cavity before vitrification. Hum Reprod 17(3):744–751
Mukaida T, Oka C et al (2006) Artificial shrinkage of blastocoeles using either a micro-needle or a laser pulse prior to the cooling steps of vitrification improves survival rate and pregnancy outcome of vitrified human blastocysts. Hum Reprod 21(12):3246–3252
Zhang X, Trokoudes K et al (2009) Vitrification of biopsied embryos at cleavage, morula and blastocyst stage. RBM Online 19(4):526–531
Son W-Y, Yoon S-H et al (2003) Pregnancy outcome following transfer of human blastocysts vitrified on electron microscopy grids after induced collapse of the blastocoele. Hum Reprod 18(1):137–139
Hardarson T et al (2006) Vitrification and warming human blastocysts by use of a laser to artificially induce blastocyst collapse prior to vitrification. Acta Obstet Gynecol Scand 86:119–120
Wikland M, Hardarson T et al (2010) Obstetric outcomes after transfer of vitrified blastocysts. Hum Reprod 27(7):1699–1707
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Laessker, A.N., Hardarsson, T., Forsberg, AS., Mukaida, T., Holmes, P.V. (2017). Appendix G: Vitrification of Blastocysts Using VitriBlastTM and ThermoBlastTM: Nidacon. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) Cryopreservation of Mammalian Gametes and Embryos. Methods in Molecular Biology, vol 1568. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6828-2_27
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6828-2_27
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6826-8
Online ISBN: 978-1-4939-6828-2
eBook Packages: Springer Protocols