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Appendix F: Quinn’s Advantage Embryo Freeze Kit

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Cryopreservation of Mammalian Gametes and Embryos

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1568))

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Abstract

Despite a large focus on the use of vitrification to cryopreserve embryos in recent years, there are still arguments for the use of slow freezing for the cleavage-stage embryo. Having said this, there are lessons to be learned from the process of vitrification that could be applied to slow freezing to improve post-thaw survival and ultimately clinical pregnancy rates. Specifically, increasing the concentration of sucrose in the freezing solution from 0.1 to 0.2 M and subsequently increasing the sucrose concentrations in thawing solutions could prove beneficial. The use of vitrification warming solutions in the thawing of slow-frozen embryos may also be an option that not only improves survival but also streamlines product purchasing and protocols within the laboratory.

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Author information

Authors and Affiliations

  1. Monash University, 3168, VIC, Melbourne, Australia

    Kiri Beilby

  2. Quinn’s IVF Consulting, 3700 S Ironwood Dr. Lot 139, Apache Junction, AZ, 85120, USA

    Patrick Quinn

Authors
  1. Kiri Beilby
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  2. Patrick Quinn
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Correspondence to Kiri Beilby .

Editor information

Editors and Affiliations

  1. Reproductive Biology Associates, Atlanta, Georgia, USA

    Zsolt Peter Nagy

  2. ASTRA Fertility Group, Mississauga, Ontario, Canada

    Alex C. Varghese

  3. Cleveland Clinic Foundation, Cleveland, Ohio, USA

    Ashok Agarwal

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Beilby, K., Quinn, P. (2017). Appendix F: Quinn’s Advantage Embryo Freeze Kit. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) Cryopreservation of Mammalian Gametes and Embryos. Methods in Molecular Biology, vol 1568. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6828-2_26

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  • DOI: https://doi.org/10.1007/978-1-4939-6828-2_26

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6826-8

  • Online ISBN: 978-1-4939-6828-2

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