Abstract
In the last 15–20 years, many centers are using vitrification as a method of choice for cryopreservation of human oocytes and embryos. As vitrification technologies have improved their success profiles, new applications seem to have emerged, making IVF treatments more successful and more flexible.
This appendix describes the Cryotech® method, which is the latest “minimal volume approach” method, suitable for cryopreservation of oocytes and embryos of any developmental stage, including blastocysts. Dr. Masashige Kuwayama, who has introduced major advances in oocyte and embryo cryopreservation, has developed this method. A detailed protocol has been described with finer tips for the accurate use of the method for perfect survival and safety.
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References
Chian RC, Kuwayama M, Tan L, Tan J, Kato O, Nagai T (2004) High survival rate of bovine oocytes matured in vitro following vitrification. J Reprod Dev 50(6):685–696
Katayama KP, Stehlik J, Kuwayama M, Kato O, Stehlik E (2003) High survival rate of vitrified human oocytes results in clinical pregnancy. Fertil Steril 80(1):223–224
Loutradi KE, Kolibianakis EM, Venetis CA, Papanikolaou EG, Pados G, Bontis I, Tarlatzis BC (2008) Cryopreservation of human embryos by vitrification or slow freezing: a systematic review and meta-analysis. Fertil Steril 90(1):186–193 Epub 2007 Nov 5
Delvigne A, Rozenberg S (2002) Epidemiology and prevention of ovarian hyperstimulation syndrome (OHSS): a review. Hum Reprod Update 8:559–577
Amso NN, Ahuja KK, Morris N, Shaw RW (1990) The management of predicted ovarian hyperstimulation involving gonadotropin-releasing hormone analog with elective cryopreservation of all pre-embryos. Fertil Steril 53(6):1087–1090
Gandhi GN, Allahbadia GN, Kagalwala S, Khatoon A, Hinduja R, Allahbadia A (2014) IVF Lite—a new strategy for managing poor ovarian responders. IVF Lite 1:22–28
Tal L, Shir D, Ekaterina S et al (2014) Comparison of IVF outcomes between minimal stimulation and high-dose stimulation for patients with poor ovarian reserve. Int J Reprod Med 2014:581451. doi:10.1155/2014/581451
Baker TG (1963) A quantitative and cytological study of germ cells in human ovaries. Proc R Soc Lond B Biol Sci 158:417–433
Martino A, Songsasen N, Leibo SP (1996) Development into blastocysts of bovine oocytes cryopreserved by ultra-rapid cooling. Biol Reprod 54(5):1059–1569
Kuwayama M (2007) Highly efficient vitrification for cryopreservation of human oocytes and embryos: the Cryotop method. Theriogenology 67(1):73–80 Epub 2006 Oct 20
Kuwayama M, Ieda S, Zhang J, Kato O (2005) The CryoTip method: aseptic vitrification of oocytes and embryos. Fertil Steril 84(Suppl 1):S187 (abstract 98)
Kagalwala S, Gandhi G, Allahbadia G, Kuwayama M, Allahbadia A, Chipkar V et al (2013) A comparison of two different vitrification methods for cryopreservation of mature human oocytes. Hum Reprod 28(1):i149–i206
Li R, Trounson A (1991) Rapid freezing of the mouse blastocyst: effects of cryoprotectants and of time and temperature of exposure to cryoprotectant before direct plunging into liquid nitrogen. Reprod Fertil Dev 3:175–183
Kolibianakis EM, Venetis CA, Tarlatzis BC (2009) Cryopreservation of human embryos by vitrification or slow freezing: which one is better? Curr Opin Obstet Gynecol 21:270–274
Mori C, Yabuuchi A, Ezoe K, Murata N, Takayama Y, Okimura T, Uchiyama K, Takakura K, Abe H, Wada K, Okuno T, Kobayashi T, Kato K (2015) Hydroxypropyl cellulose as an option for supplementation of cryoprotectant solutions for embryo vitrification in human assisted reproductive technologies. Reprod Biomed Online 30(6):613–621
Kuwayama M, Vajta G, Kato O, Leibo SP (2005) Highly efficient vitrification method for cryopreservation of human oocytes. Reprod Biomed Online 11(3):300–308
Seki S, Mazur P (2009) The dominance of warming rate over cooling rate in the survival of mouse oocytes subjected to a vitrification procedure. Cryobiology 59(1):75–82
Pomeroy KO, Harris S, Conaghan J, Papadakis M, Centola G, Basuray R, Battaglia D (2010) Storage of cryopreserved reproductive tissues: evidence that cross-contamination of infectious agents is a negligible risk. Fertil Steril 94(4):1181–1188
Bielanski A, Vajta G (2009) Risk of contamination of germplasm during cryopreservation and cryobanking in IVF units. Hum Reprod 24(10):2457–2467
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Gandhi, G., Kuwayama, M., Kagalwala, S., Pangerkar, P. (2017). Appendix A: Cryotech® Vitrification Thawing. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) Cryopreservation of Mammalian Gametes and Embryos. Methods in Molecular Biology, vol 1568. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6828-2_21
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DOI: https://doi.org/10.1007/978-1-4939-6828-2_21
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