Skip to main content

Chapter 14 Establishing an Oocyte Cryobank Network

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1568))

Abstract

Improvements in oocyte cryopreservation has prompted wider acceptance of this technology leading to its use for several reasons. Notably, in addition to elective and medically driven reasons for oocyte cryostorage for fertility preservation, donor oocyte cryobanking is beginning to gain traction, potentially replacing fresh oocyte donation in assisted reproduction. Donor “egg banking,” while not totally analogous to donor sperm banking, does provide strong benefits in terms of scheduling flexibility and improved clinical efficiencies, while providing a wider immediate inventory choice. The development of a successful cryobank “network” and subsequent growth into a full-access donor egg bank are only possible through adoption of a series of key steps involving establishment of a repeatable vitrification protocol with a strong clinical record, incorporation of a comprehensive database and quality management system, and strict control over the logistics of inventory and shipping and receiving, to establish a flawless chain from donor to recipient. Confidence will grow in this potentially difficult process over time.

This is a preview of subscription content, log in via an institution.

Buying options

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   159.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Tucker MJ, Morton PC, Wright G, Sweitzer CL, Massey JB (1998) Clinical application of human egg cryopreservation. Hum Reprod 13:3156–3159

    Article  CAS  PubMed  Google Scholar 

  2. Tucker M, Morton P, Liebermann J (2004) Human oocyte cryopreservation: a valid alternative to embryo cryopreservation? Eur J Obstet Gynecol Reprod Biol 113S:S24–S27

    Article  Google Scholar 

  3. Cobo A, Meseguer M, Remohi J et al (2010) Use of cryobanked oocytes in an ovum donation programme: a prospective, randomized, controlled, clinical trial. Hum Reprod 25:2239–2246

    Article  PubMed  Google Scholar 

  4. Nagy ZP, Chang CC, Shapiro DB et al (2009) Clinical evaluation of the efficiency of an oocyte donation program using egg cryo-banking. Fertil Steril 92:520–526

    Article  PubMed  Google Scholar 

  5. Larman M et al RBM Online 2007 Citation, Borini A, Lagalla C, Bonu MA et al (2006) Clinical outcome of oocyte cryopreservation after slow cooling with a protocol utilizing a high sucrose concentration. Hum Reprod 21:512–517

    Google Scholar 

  6. De Santis L, Cino I, Rabellotti E et al (2007) Oocyte cryopreservation: clinical outcome of slow-cooling protocols differing in sucrose concentration. Reprod Biomed Online 14:57–63

    Article  CAS  PubMed  Google Scholar 

  7. Borini A, Lagalla C, Bonu MA et al (2006) Clinical outcome of oocyte cryopreservation after slow cooling with a protocol utilizing a high sucrose concentration. Hum Reprod 21:512–517

    Article  CAS  PubMed  Google Scholar 

  8. Cobo A, Kuwayama M, Perez S et al (2008) Comparison of concomitant outcome achieved with fresh and cryopreserved donor oocytes vitrified by the cryotop method. Fertil Steril 89:1657–1664

    Article  PubMed  Google Scholar 

  9. Cobo A, Diaz C (2011) Clinical application of oocyte vitrification: a systematic review and meta-analysis of randomized controlled trials. Fertil Steril 96:277–285

    Article  PubMed  Google Scholar 

  10. Bianchi V, Lappi M, MA B et al (2012) Oocyte slow freezing using a 0.2-0.3M sucrose concentration protocol: is it really the time to trash the cryopreservation machine? Fertil Steril 97:1101–1107

    Article  CAS  PubMed  Google Scholar 

  11. Cil AP, Bang H, Oktay K (2013) Age-specific probability of live birth with oocyte cryopreservation: an individual patient data meta-analysis. Fertil Steril 100:492

    Article  PubMed  PubMed Central  Google Scholar 

  12. Cobo A, Perez S, MJ D l S, Zulatequi J, Domingo J, Remohi J Effect of different cryopreservation techniques on the metaphase II spindle in human oocytes. Reprod Biomed Online 17:350–359

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding authors

Correspondence to James Graham , Josh Lim or Michael Tucker .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer Science+Business Media LLC

About this protocol

Cite this protocol

Graham, J., Lim, J., Tucker, M. (2017). Chapter 14 Establishing an Oocyte Cryobank Network. In: Nagy, Z., Varghese, A., Agarwal, A. (eds) Cryopreservation of Mammalian Gametes and Embryos. Methods in Molecular Biology, vol 1568. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6828-2_14

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-6828-2_14

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6826-8

  • Online ISBN: 978-1-4939-6828-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics