Abstract
Lipids draw increasing attention of cell biologists because of the wide variety of functions beyond their role as building blocks of cellular membranes. Mitochondrial membranes possess characteristic lipid compositions that are intimately associated with mitochondrial architecture and activities. Therefore, quantitative assessment of lipids in isolated mitochondria is of importance for mitochondrial research. Here, I describe our workflow for quantitative analysis of glycerophospholipids in mitochondria with a focus on purification of pure mitochondrial fractions from yeast and cultured mammalian cells as well as improved settings for the analysis of cardiolipin by nano-electrospray ionization mass spectrometry.
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Acknowledgments
I thank Shotaro Saita and Kavia Bakka for providing protocols and example results, Susanne Brodesser and Mari Aaltonen for critical reading of the manuscript, and Thomas Langer for continuous support. I also acknowledge S. B. and Matthias Haag for technical advices in lipidomics. This work is supported by a grant of the Deutsche Forschungsgemeinschaft (TA 1132/2-1).
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Tatsuta, T. (2017). Quantitative Analysis of Glycerophospholipids in Mitochondria by Mass Spectrometry. In: Mokranjac, D., Perocchi, F. (eds) Mitochondria. Methods in Molecular Biology, vol 1567. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6824-4_7
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DOI: https://doi.org/10.1007/978-1-4939-6824-4_7
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