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Isolation of Mitochondria from Saccharomyces cerevisiae

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Mitochondria

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1567))

Abstract

The budding yeast Saccharomyces cerevisiae is an important model organism to study cellular structure and function. Due to its excellent accessibility to genetics and biochemical and microscopic analyses, studies with yeast have provided fundamental insights into mitochondrial biology. Yeast offers additional advantages because it can grow under fermenting conditions when oxidative phosphorylation is not obligatory and because the majority of mitochondrial structure and function are largely conserved during evolution. Isolation of mitochondria is an important technique for mitochondrial studies. This chapter focuses on procedures for the isolation and purification of intact yeast mitochondria that can be used for numerous functional assays as well as for analyses of mitochondrial ultrastructure.

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References

  1. Gancedo JM (1998) Yeast carbon catabolite repression. Microbiol Mol Biol Rev 62(2):334–361

    CAS  PubMed  PubMed Central  Google Scholar 

  2. Egner A, Jakobs S, Hell SW (2002) Fast 100-nm resolution three-dimensional microscope reveals structural plasticity of mitochondria in live yeast. Proc Natl Acad Sci U S A 99(6):3370–3375

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Visser W, van Spronsen EA, Nanninga N, Pronk JT, Gijs Kuenen J, van Dijken JP (1995) Effects of growth conditions on mitochondrial morphology in Saccharomyces cerevisiae. Antonie Van Leeuwenhoek 67(3):243–253

    Article  CAS  PubMed  Google Scholar 

  4. Harner M, Körner C, Walther D, Mokranjac D, Kaesmacher J, Welsch U, Griffith J, Mann M, Reggiori F, Neupert W (2011) The mitochondrial contact site complex, a determinant of mitochondrial architecture. EMBO J 30(21):4356–4370

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Yamamoto H, Esaki M, Kanamori T, Tamura Y, Nishikawa S, Endo T (2002) Tim50 Is a subunit of the TIM23 complex that links protein translocation across the outer and inner mitochondrial membranes. Cell 111(4):519–528

    Article  CAS  PubMed  Google Scholar 

  6. Mokranjac D, Mokranjac D, Paschen SA, Kozany C, Prokisch H, Hoppins SC, Nargang FE, Neupert W, Hell K (2003) Tim50, a novel component of the TIM23 preprotein translocase of mitochondria. EMBO J 22(4):816–825

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Geissler A, Chacinska A, Truscott KN, Wiedemann N, Brandner K, Sickmann A, Meyer HE, Meisinger C, Pfanner N, Rehling P (2002) Mitochondrial presequence translocase: an essential role of Tim50 in directing preproteins to the import channel. Cell 111(4):507–518

    Article  CAS  PubMed  Google Scholar 

  8. Daum G, Böhni PC, Schatzg G (1982) Import of proteins into mitochondria. Cytochrome b2 and cytochrome c peroxidase are located in the intermembrane space of yeast mitochondria. J Biol Chem 257(21):13034–13041

    PubMed  Google Scholar 

  9. Meisinger C, Sommer T, Pfanner N (2000) Purification of saccharomcyes cerevisiae mitochondria devoid of microsomal and cytosolic contaminations. Anal Biochem 287(2):339–342

    Article  CAS  PubMed  Google Scholar 

  10. Altmann K, Dürr M, Westermann B (2007) Saccharomyces cerevisiae as a model organism to study mitochondrial biology: general considerations and basic procedures. Methods Mol Biol 372:81–90

    Article  CAS  PubMed  Google Scholar 

  11. Lee AC, Xu X, Blachly-Dyson E, Forte M, Colonbini M (1998) The role of yeast VDAC genes on the permeability of the mitochondrial outer membrane. J Membr Biol 161(2):173–181

    Article  CAS  PubMed  Google Scholar 

  12. Cabib E, Roberts R, Bowers B (1982) Synthesis of the yeast cell wall and its regulation. Annu Rev Biochem 51:763–793

    Article  CAS  PubMed  Google Scholar 

  13. Kitamura K, Kaneko T, Yamamoto Y (1971) Lysis of viable yeast cells by enzymes of arfhrobacfer luteus. Arch Biochem Biophys 145(1):402–404

    Article  CAS  PubMed  Google Scholar 

  14. Kitamura K (1982) Re-examination of zymolyase purification. Agric Biol Chem 46(4):963–969

    CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152, Martinsried, Germany

    Toshiaki Izawa

  2. Cell Biology and Electron Microscopy, Institut für Zellbiologie, Universität Bayreuth, 95440, Bayreuth, Germany

    Ann-Katrin Unger

Authors
  1. Toshiaki Izawa
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  2. Ann-Katrin Unger
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Corresponding author

Correspondence to Toshiaki Izawa .

Editor information

Editors and Affiliations

  1. Biomedical Center-Physiological Chemistry, LMU Munich, Martinsried, Germany

    Dejana Mokranjac

  2. Gene Center, LMU Munich and Institute for Diabetes and Obesity, Helmholtz Zentrum München, Munich, Germany

    Fabiana Perocchi

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Izawa, T., Unger, AK. (2017). Isolation of Mitochondria from Saccharomyces cerevisiae . In: Mokranjac, D., Perocchi, F. (eds) Mitochondria. Methods in Molecular Biology, vol 1567. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6824-4_3

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  • DOI: https://doi.org/10.1007/978-1-4939-6824-4_3

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6822-0

  • Online ISBN: 978-1-4939-6824-4

  • eBook Packages: Springer Protocols

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