Abstract
Adipose tissue plays an important role in whole body metabolism, and its dysfunction results in poor metabolic health and is associated with a diverse set of disorders, such as obesity, cardiovascular disease, hypertension, cancer, and type 2 diabetes. The search for cellular processes that can be targeted to activate adipose oxidative metabolism is thought to hold promise for combating these diseases, and so the analysis of adipose bioenergetics is crucial toward this end. A description is provided here for the preparation of mitochondria and primary adipocytes from adipose tissue to be used for bioenergetics profiling.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Chance B, Williams GR (1956) The respiratory chain and oxidative phosphorylation. Adv Enzymol Relat Subj Biochem 17:65–134
Brand MD, Nicholls DG (2011) Assessing mitochondrial dysfunction in cells. Biochem J 435(2):297–312. doi:10.1042/BJ20110162
Gerencser AA, Neilson A, Choi SW, Edman U, Yadava N, Oh RJ, Ferrick DA, Nicholls DG, Brand MD (2009) Quantitative microplate-based respirometry with correction for oxygen diffusion. Anal Chem 81(16):6868–6878. doi:10.1021/ac900881z
Kazak L, Chouchani ET, Jedrychowski MP, Erickson BK, Shinoda K, Cohen P, Vetrivelan R, Lu GZ, Laznik-Bogoslavski D, Hasenfuss SC, Kajimura S, Gygi SP, Spiegelman BM (2015) A creatine-driven substrate cycle enhances energy expenditure and thermogenesis in beige fat. Cell 163(3):643–655. doi:10.1016/j.cell.2015.09.035
He J, Mao CC, Reyes A, Sembongi H, Di Re M, Granycome C, Clippingdale AB, Fearnley IM, Harbour M, Robinson AJ, Reichelt S, Spelbrink JN, Walker JE, Holt IJ (2007) The AAA+ protein ATAD3 has displacement loop binding properties and is involved in mitochondrial nucleoid organization. J Cell Biol 176(2):141–146. doi:10.1083/jcb.200609158
Yang MY, Bowmaker M, Reyes A, Vergani L, Angeli P, Gringeri E, Jacobs HT, Holt IJ (2002) Biased incorporation of ribonucleotides on the mitochondrial L-strand accounts for apparent strand-asymmetric DNA replication. Cell 111(4):495–505
Nicholls DG, Grav HJ, Lindberg O (1972) Mitochondrial from hamster brown-adipose tissue. Regulation of respiration in vitro by variations in volume of the matrix compartment. Eur J Biochem/FEBS 31(3):526–533
Parker N, Crichton PG, Vidal-Puig AJ, Brand MD (2009) Uncoupling protein-1 (UCP1) contributes to the basal proton conductance of brown adipose tissue mitochondria. J Bioenerg Biomembr 41(4):335–342. doi:10.1007/s10863-009-9232-8
Li Y, Fromme T, Schweizer S, Schottl T, Klingenspor M (2014) Taking control over intracellular fatty acid levels is essential for the analysis of thermogenic function in cultured primary brown and brite/beige adipocytes. EMBO Rep 15(10):1069–1076. doi:10.15252/embr.201438775
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2017 Springer Science+Business Media LLC
About this protocol
Cite this protocol
Kazak, L. (2017). Bioenergetic Analyses in Adipose Tissue. In: Wu, J. (eds) Thermogenic Fat. Methods in Molecular Biology, vol 1566. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6820-6_12
Download citation
DOI: https://doi.org/10.1007/978-1-4939-6820-6_12
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-6819-0
Online ISBN: 978-1-4939-6820-6
eBook Packages: Springer Protocols