Abstract
Photoactivatable morpholinos (MO) allow specific temporal and spatial inhibition of gene function, which is not possible with conventional morpholino or genetic global gene knock-out approaches. Here, we describe an application of commercially available photoactivatable MO technology for specific gene inhibition in a zebrafish embryonic model and discuss the required controls related to the specificity and efficacy of this method. A similar approach should be also applicable to other model organisms.
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Acknowledgment
This work was supported by NIH grant HL107369 and Cincinnati Children’s Research Foundation.
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Sumanas, S. (2017). Inducible Inhibition of Gene Function with Photomorpholinos. In: Moulton, H., Moulton, J. (eds) Morpholino Oligomers. Methods in Molecular Biology, vol 1565. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6817-6_5
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DOI: https://doi.org/10.1007/978-1-4939-6817-6_5
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6817-6
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