Abstract
Brassinosteroids (BRs) are a class of plant steroidal hormones that play essential roles in plant growth and development. Systematic studies had first been undertaken concomitantly to determine both the effects of exogenous BR on stress phenotypes of Arabidopsis thaliana and Brassica napus (rapeseed) seedlings and the expression of stress marker genes in BR-treated and untreated seedlings. When reproducible and convincing evidence of the role of BR in stress tolerance had been obtained, molecular mechanisms underlying the ability of BR to confer tolerance against heat, cold, drought, and salt stress, as well as pathogen resistance were studied with several molecular approaches and tools. The results of these studies have together provided valuable insights into how BRs, through their control of many basic cellular processes and stress responses, promote vigor in plants and prepare the plant to mount a dynamic response upon environmental challenges. Protocols to assess BR effects on abiotic stress tolerance in Arabidopsis and rapeseed seedlings are described here and they can be fine-tuned and adapted for other plant species.
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References
Clouse SD (2011) Brassinosteroids. The Arabidopsis Book 9:e0151
Yang C-J, Zhang C, Lu Y-N et al (2011) The mechanisms of brassinosteroids’ action: from signal transduction to plant development. Mol Plant 4:588–600
Zhu J-Y, Sae-Seaw J, Wang Z-Y (2013) Brassinosteroid signalling. Development 140:1615–1620
Belkhadir Y, Jaillais Y (2015) The molecular circuitry of brassinosteroid signalling. New Phytol 206:522–540
Krishna P (2003) Brassinosteroid-mediated stress responses. J Plant Growth Regul 22:289–297
Divi UK, Krishna P (2010) Brassinosteroids confer stress tolerance. In: Hirt H (ed) Plant stress biology: from genomics to system biology. Wiley, New York, pp 119–135
Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assay with tobacco tissue cultures. Physiol Plant 15:473–497
Dhaubhadel S, Chaudhary S, Dobinson KF et al (1999) Treatment with 24-epibrassinolide, a brassinosteroid, increases the basic thermotolerance of Brassica napus and tomato seedlings. Plant Mol Biol 40:333–342
Divi UK, Rahman T, Krishna P (2010) Brassinosteroid-mediated stress tolerance in Arabidopsis shows interactions with abscisic acid, ethylene and salicylic acid pathways. BMC Plant Biol 10:151
Dhaubhadel S, Browning KS, Gallie DR et al (2002) Brassinosteroid functions to protect the translational machinery and heat-shock protein synthesis following thermal stress. Plant J 29:681–691
Divi UK, Rahman T, Krishna P (2016) Gene expression and functional analyses in brassinosteroid-mediated stress tolerance. Plant Biotechnol J 14:419–432
Liu Y, Jiang H, Zhao Z et al (2011) Abscisic acid is involved in brassinosteroids-induced chilling tolerance in the suspension cultured cells from Chorispora bungeana. J Plant Physiol 168:853–862
Deng X-G, Zhu T, Zhang D-W et al (2015) The alternative respiratory pathway is involved in brassinosteroid-induced environmental stress tolerance in Nicotiana benthamiana. J Exp Bot 66:6219–6232
Wei L-J, Deng X-G, Zhu T et al (2015) Ethylene is involved in brassinosteroids induced alternative respiratory pathway in cucumber (Cucumis sativus L.) seedlings response to abiotic stress. Front Plant Sci 6:982
Gao H, Kang L, Liu Q et al (2015) Effect of 24-epibrassinolide treatment on the metabolism of eggplant fruits in relation to development of pulp browning under chilling stress. J Food Sci Technol 52:3394–3401
Divi UK, Krishna P (2010) Overexpression of the brassinosteroid biosynthetic gene AtDWF4 in Arabidopsis seeds overcomes abscisic acid-induced inhibition of germination and increases cold tolerance in transgenic seedlings. J Plant Growth Regul 29:385–393
Saha G, Park J-I, Jung H-J et al (2015) Molecular characterization of BZR transcription factor family and abiotic stress induced expression profiling in Brassica rapa. Plant Physiol Biochem 92:92–104
Kagale S, Divi UK, Krochko J et al (2007) Brassinosteroid confers tolerance in Arabidopsis thaliana and Brassica napus to a range of abiotic stresses. Planta 225:353–364
Sahni S, Prasad BD, Liu Q et al (2016) Overexpression of the brassinosteroid biosynthetic gene DWF4 in Brassica napus simultaneously increases seed yield and stress tolerance. Sci Rep 6:28298
Janeczko A, Gruszka D, Pociecha E et al (2015) Physiological and biochemical characterisation of watered and drought-stressed barley mutants in the HvDWARF gene encoding C6-oxidase involved in brassinosteroid biosynthesis. Plant Physiol Biochem 99:126–141
Zhu T, Deng X-G, Tan W-R et al (2015) Nitric oxide is involved in brassinosteroid-induced alternative respiratory pathway in Nicotiana benthamiana seedlings’ response to salt stress. Physiol Plant 156:150–163
Sharma I, Ching E, Saini S et al (2013) Exogenous application of brassinosteroid offers tolerance to salinity by altering stress responses in rice variety Pusa Basmati-1. Plant Physiol Biochem 69:17–26
Cui F, Liu L, Zhao Q et al (2012) Arabidopsis ubiquitin conjugase UBC32 is an ERAD component that functions in brassinosteroid-mediated salt stress tolerance. Plant Cell 24:233–244
Li Z-Y, Xu Z-S, He G-Y et al (2012) A mutation in Arabidopsis BSK5 encoding a brassinosteroid-signalling kinase protein affects responses to salinity and abscisic acid. Biochem Biophys Res Commun 426:522–527
Yan J, Guan L, Sun Y et al (2015) Calcium and ZmCCaMK are involved in brassinosteroid-induced antioxidant defense in maize leaves. Plant Cell Physiol 56:883–896
Zhang A et al (2010) ZmMPK5 is required for the NADPH oxidase-mediated self-propagation of apoplastic H2O2 in brassinosteroid-induced antioxidant defence in leaves of maize. J Exp Bot 61(15):4399–4411
Acknowledgements
We thank the Natural Sciences and Engineering Research Council of Canada for supporting the studies and research personnel. B.D.P. was supported through a Postdoctoral Doctoral Fellowship, and T.R. through Ph.D. Research and Teaching Assistantships.
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Krishna, P., Prasad, B.D., Rahman, T. (2017). Brassinosteroid Action in Plant Abiotic Stress Tolerance. In: Russinova, E., Caño-Delgado, A. (eds) Brassinosteroids. Methods in Molecular Biology, vol 1564. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6813-8_16
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DOI: https://doi.org/10.1007/978-1-4939-6813-8_16
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