Targeted Ablation Using Laser Nanosurgery

  • Naga Venkata Gayathri Vegesna
  • Paolo Ronchi
  • Sevi Durdu
  • Stefan Terjung
  • Rainer PepperkokEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1563)


Laser-mediated dissection methods have been used for many years to micro-irradiate biological samples, but recent technological progress has rendered this technique more precise, powerful, and easy to use. Today pulsed lasers can be operated with diffraction limited, sub-micrometer precision to ablate intracellular structures. Here, we discuss laser nanosurgery setups and the instrumentation in our laboratory. We describe how to use this technique to ablate cytoskeletal elements in living cells. We also show how this technique can be used in multicellular organisms, to micropuncture and/or ablate cells of interest and finally how to monitor a successful laser nanosurgery.

Key words

Laser nanosurgery Subcellular structures Laser ablation Micropuncture Photobleach 


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Naga Venkata Gayathri Vegesna
    • 1
  • Paolo Ronchi
    • 1
    • 2
  • Sevi Durdu
    • 1
  • Stefan Terjung
    • 3
  • Rainer Pepperkok
    • 1
    • 3
    Email author
  1. 1.Cell Biology and Cell Biophysics UnitEMBL HeidelbergHeidelbergGermany
  2. 2.Electron Microscopy Core FacilityEMBL HeidelbergHeidelbergGermany
  3. 3.Advanced Light Microscopy FacilityEMBL HeidelbergHeidelbergGermany

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