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Targeted Ablation Using Laser Nanosurgery

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Light Microscopy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1563))

Abstract

Laser-mediated dissection methods have been used for many years to micro-irradiate biological samples, but recent technological progress has rendered this technique more precise, powerful, and easy to use. Today pulsed lasers can be operated with diffraction limited, sub-micrometer precision to ablate intracellular structures. Here, we discuss laser nanosurgery setups and the instrumentation in our laboratory. We describe how to use this technique to ablate cytoskeletal elements in living cells. We also show how this technique can be used in multicellular organisms, to micropuncture and/or ablate cells of interest and finally how to monitor a successful laser nanosurgery.

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Author information

Authors and Affiliations

  1. Cell Biology and Cell Biophysics Unit, EMBL Heidelberg, Meyerhofstrasse 1, 69117, Heidelberg, Germany

    Naga Venkata Gayathri Vegesna, Paolo Ronchi, Sevi Durdu & Rainer Pepperkok

  2. Electron Microscopy Core Facility, EMBL Heidelberg, Meyerhofstrasse 1, 69117, Heidelberg, Germany

    Paolo Ronchi

  3. Advanced Light Microscopy Facility, EMBL Heidelberg, Meyerhofstrasse 1, 69117, Heidelberg, Germany

    Stefan Terjung & Rainer Pepperkok

Authors
  1. Naga Venkata Gayathri Vegesna
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  2. Paolo Ronchi
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  3. Sevi Durdu
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  4. Stefan Terjung
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  5. Rainer Pepperkok
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Corresponding author

Correspondence to Rainer Pepperkok .

Editor information

Editors and Affiliations

  1. Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, California, USA

    Yolanda Markaki

  2. Biozentrum der LMU Munchen, Planegg-Martinsried, Germany

    Hartmann Harz

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Gayathri Vegesna, N.V., Ronchi, P., Durdu, S., Terjung, S., Pepperkok, R. (2017). Targeted Ablation Using Laser Nanosurgery. In: Markaki, Y., Harz, H. (eds) Light Microscopy. Methods in Molecular Biology, vol 1563. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6810-7_8

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  • DOI: https://doi.org/10.1007/978-1-4939-6810-7_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6808-4

  • Online ISBN: 978-1-4939-6810-7

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