FRET Microscopy for Real-Time Visualization of Second Messengers in Living Cells
Förster Resonance Energy Transfer (FRET) microscopy is a useful tool in molecular biology and medical research to monitor and quantify real-time dynamics of protein-protein interactions and biochemical processes. Using this well-established technique, many novel signaling mechanisms can be investigated in intact cells or tissues and even in various subcellular compartments. Here, we describe how to perform FRET measurements in living cells expressing FRET-based biosensors and how to evaluate these data. This general protocol can be applied for FRET measurements with various fluorescent biosensors.
Key wordsFRET Microscopy Imaging Fluorescence Biosensor
The work in authors’ laboratory is supported by the Deutsche Forschungsgemeinschaft (grants NI 1301/1, FOR 2060), DZHK, and the Gertraud und Heinz Rose-Stiftung.
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