Imaging of Brain Slices with a Genetically Encoded Voltage Indicator
Functional fluorescence microscopy of brain slices using voltage sensitive fluorescent proteins (VSFPs) allows large scale electrophysiological monitoring of neuronal excitation and inhibition. We describe the equipment and techniques needed to successfully record functional responses optical voltage signals from cells expressing a voltage indicator such as VSFP Butterfly 1.2. We also discuss the advantages of voltage imaging and the challenges it presents.
Key wordsVoltage imaging Fluorescence imaging Brain slices Voltage indicators Fluorescence microscopy Voltage sensitive fluorescent proteins
This work was supported by the Engineering and Physical Sciences Research Council [grant number EP/L016737/1]. We would like to thank Elisa Ciglieri, Amanda Foust, Taylor Lyons, and Chenchen Song for their very helpful comments and advice on the manuscript.