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Imaging of Brain Slices with a Genetically Encoded Voltage Indicator

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Light Microscopy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1563))

Abstract

Functional fluorescence microscopy of brain slices using voltage sensitive fluorescent proteins (VSFPs) allows large scale electrophysiological monitoring of neuronal excitation and inhibition. We describe the equipment and techniques needed to successfully record functional responses optical voltage signals from cells expressing a voltage indicator such as VSFP Butterfly 1.2. We also discuss the advantages of voltage imaging and the challenges it presents.

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Acknowledgments

This work was supported by the Engineering and Physical Sciences Research Council [grant number EP/L016737/1]. We would like to thank Elisa Ciglieri, Amanda Foust, Taylor Lyons, and Chenchen Song for their very helpful comments and advice on the manuscript.

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Authors and Affiliations

  1. Department of Bioengineering, Imperial College London, South Kensington, London, SW7 2AZ, UK

    Peter Quicke

  2. Centre for Neurotechnology, Imperial College London, South Kensington, London, SW7 2AZ, UK

    Peter Quicke & Thomas Knöpfel

  3. Division of Brain Sciences, Imperial College London, Hammersmith Campus, London, W12 0NN, UK

    Peter Quicke, Samuel J. Barnes & Thomas Knöpfel

Authors
  1. Peter Quicke
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  2. Samuel J. Barnes
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  3. Thomas Knöpfel
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Corresponding author

Correspondence to Thomas Knöpfel .

Editor information

Editors and Affiliations

  1. Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, California, USA

    Yolanda Markaki

  2. Biozentrum der LMU Munchen, Planegg-Martinsried, Germany

    Hartmann Harz

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Quicke, P., Barnes, S.J., Knöpfel, T. (2017). Imaging of Brain Slices with a Genetically Encoded Voltage Indicator. In: Markaki, Y., Harz, H. (eds) Light Microscopy. Methods in Molecular Biology, vol 1563. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6810-7_5

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  • DOI: https://doi.org/10.1007/978-1-4939-6810-7_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6808-4

  • Online ISBN: 978-1-4939-6810-7

  • eBook Packages: Springer Protocols

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