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Fluorescence-Based High-Throughput and Targeted Image Acquisition and Analysis for Phenotypic Screening

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Light Microscopy

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1563))

Abstract

Applying the right acquisition method in a fluorescence imaging-based screening context is of great importance to obtain an appropriate readout and to select the right scale of the screen. In order to save imaging time and data, we have developed routines for multiscale targeted imaging, providing both a broad overview of a sample and additional in-depth information for targets of interest identified within the screen. These objects can be identified and acquired on-the-fly by an interconnection of image acquisition and image analysis.

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Acknowledgment

The authors wish to thank Michael Berthold and the KNIME/KNIP developers for their help with the development of the KNIME nodes and workflows. This work was funded within the project CancerTelSys (grant number 01ZX1302) in the e:Med program, the project HD-HuB (grant number 031A537C) in the de.NBI program and within the project RNA-Code (grant number 031A298) in the e:Bio program, all of the German Federal Ministry of Education and Research (BMBF). The ViroQuant-CellNetworks RNAi Screening Facility was also supported by the CellNetworks-Cluster of Excellence (grant number EXC81).

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Authors and Affiliations

  1. BioQuant Center, ViroQuant-CellNetworks RNAi Screening Facility, University of Heidelberg, Im Neuenheimer Feld 267, 69120, Heidelberg, Germany

    Manuel Gunkel, Jan Philipp Eberle & Holger Erfle

Authors
  1. Manuel Gunkel
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  2. Jan Philipp Eberle
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  3. Holger Erfle
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Corresponding author

Correspondence to Manuel Gunkel .

Editor information

Editors and Affiliations

  1. Department of Biological Chemistry, UCLA School of Medicine, Los Angeles, California, USA

    Yolanda Markaki

  2. Biozentrum der LMU Munchen, Planegg-Martinsried, Germany

    Hartmann Harz

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Gunkel, M., Eberle, J.P., Erfle, H. (2017). Fluorescence-Based High-Throughput and Targeted Image Acquisition and Analysis for Phenotypic Screening. In: Markaki, Y., Harz, H. (eds) Light Microscopy. Methods in Molecular Biology, vol 1563. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6810-7_17

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  • DOI: https://doi.org/10.1007/978-1-4939-6810-7_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6808-4

  • Online ISBN: 978-1-4939-6810-7

  • eBook Packages: Springer Protocols

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