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Liquid Chromatography-Mass Spectrometry for Analysis of RNA Adenosine Methylation

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RNA Methylation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1562))

Abstract

Dynamic RNA modifications recently were considered to constitute another realm for biological regulation in the form of “RNA epigenetics”. N 6-methyladenosine (m6A), one of the most important modifications on RNA, plays a fundamental role in epigenetic regulation of the mammalian transcriptome. We recently established various liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS/MS)-based methods for the sensitive and accurate determination of modified nucleosides in both DNA and RNA. Here, we describe a protocol to analyze m6A in RNA by LC-ESI-MS/MS. And this protocol also can be extended to the analysis of other modified nucleosides in both DNA and RNA.

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Acknowledgments

The author thanks the financial support from the National Natural Science Foundation of China (21522507, 21672166).

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Authors and Affiliations

  1. Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, 430072, China

    Bi-Feng Yuan

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  1. Bi-Feng Yuan
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Correspondence to Bi-Feng Yuan .

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Editors and Affiliations

  1. Division of Molecular Biology, Biocenter, Medical University of Innsbruck, Innsbruck, Austria

    Alexandra Lusser

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Yuan, BF. (2017). Liquid Chromatography-Mass Spectrometry for Analysis of RNA Adenosine Methylation. In: Lusser, A. (eds) RNA Methylation. Methods in Molecular Biology, vol 1562. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6807-7_3

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  • DOI: https://doi.org/10.1007/978-1-4939-6807-7_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6805-3

  • Online ISBN: 978-1-4939-6807-7

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