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RNA Methylation pp 283–294Cite as

Methylated mRNA Nucleotides as Regulators for Ribosomal Translation

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1562))

Abstract

Methylated RNA nucleotides were recently discovered to be highly abundant in RNAs. The effects of these methylations were mainly attributed to altered mRNA stabilities, protein-binding affinities, or RNA structures. The direct impact of RNA modifications on the performance of the ribosome has not been investigated so far. In this chapter, we describe an approach that allows introducing RNA modifications site-specifically into coding sequences of mRNAs and determining their effect on the translation machinery in a well-defined bacterial in vitro system.

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Acknowledgment

This work was supported by the FWF (P 22658-B12 and P 28494-BBL to M.E.)

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Authors and Affiliations

  1. Division of Genomics and RNomics, Biocenter Innsbruck, Medical University of Innsbruck, Innrain 80/82, Innsbruck, 6020, Austria

    Thomas P. Hoernes & Matthias D. Erlacher

Authors
  1. Thomas P. Hoernes
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  2. Matthias D. Erlacher
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Corresponding author

Correspondence to Matthias D. Erlacher .

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Editors and Affiliations

  1. Division of Molecular Biology, Biocenter, Medical University of Innsbruck, Innsbruck, Austria

    Alexandra Lusser

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Hoernes, T.P., Erlacher, M.D. (2017). Methylated mRNA Nucleotides as Regulators for Ribosomal Translation. In: Lusser, A. (eds) RNA Methylation. Methods in Molecular Biology, vol 1562. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6807-7_19

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  • DOI: https://doi.org/10.1007/978-1-4939-6807-7_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6805-3

  • Online ISBN: 978-1-4939-6807-7

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