The Use of Lectin Histochemistry for Detecting Apoptotic Cells in the Seminiferous Epithelium

  • Vicente Seco-Rovira
  • Ester Beltrán-Frutos
  • Jesús Martínez-Hernández
  • Concepción Ferrer
  • Luis Miguel Pastor
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1560)

Abstract

Lectin histochemistry is commonly used to characterize the pattern of glycoconjugates in cells and tissues. Recent studies show that alterations in these glycoconjugates are associated with the entry of cells into apoptosis. A widely used technique for the detection of apoptotic cell death is TUNEL. In this chapter, we study the sensitivity of both techniques to identify apoptotic cells in the testis of photo-inhibited Syrian hamster.

Key words

Lectin histochemistry TUNEL Apoptosis Testis Germ cell Syrian hamster Sensitivity 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Vicente Seco-Rovira
    • 1
  • Ester Beltrán-Frutos
    • 1
  • Jesús Martínez-Hernández
    • 1
  • Concepción Ferrer
    • 1
  • Luis Miguel Pastor
    • 1
  1. 1.Department of Cell Biology and Histology, School of Medicine, IMIB-ArrixacaUniversity of MurciaEspinardo, MurciaSpain

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