Abstract
Determination of cellular neutral lipid levels in yeast is important for both the biotechnology industry and biomedical research. However, many of the currently available methods are labor intensive and time consuming. Here we describe a rapid and repeatable method for the detection of neutral lipids, which can be utilized in both oleaginous and non-oleaginous yeast species. The method utilizes the fluorescent dye, Nile red, which enables neutral lipid levels to either be visualized via microscopy or quantified using a 96-well plate assay.
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Acknowledgments
This work was supported by The University of Central Lancashire and Brain Tumour North West. The authors would like to acknowledge Dr. Gail Welsby and Dr. Vicky Jones for assistance with microscopy, Mr. Tony Dickson for technical assistance, and Dr. Christopher Smith for support with statistical analysis.
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Rostron, K.A., Lawrence, C.L. (2017). Nile Red Staining of Neutral Lipids in Yeast. In: Pellicciari, C., Biggiogera, M. (eds) Histochemistry of Single Molecules. Methods in Molecular Biology, vol 1560. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6788-9_16
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DOI: https://doi.org/10.1007/978-1-4939-6788-9_16
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