Abstract
Particle bombardment of gold microparticles coated with plasmids, which are accelerated to high velocity, is used for transfection of cells within tissue. Using this method, cDNA encoding proteins of interest introduced into ex vivo living human skin enables studying of proteins of interest in real time. Here, technical aspects of particle bombardment of ex vivo skin are described using green fluorescent protein (GFP) as readout for efficiency. This method can be applied on numerous tissues, including in living model animals.
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Acknowledgment
We thank Oscar Stassen and Elly Hol (Free University Amsterdam, now Utrecht University) for training. Part of the work has been performed in the UMCG Microscopy and Imaging Center (UMIC). Funding for these studies was provided by the Netherlands Organization for Scientific Research (NWO 175-010-2009-023) and the Gratama Foundation.
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Sokol, E., Nijenhuis, M., Sjollema, K.A., Jonkman, M.F., Pas, H.H., Giepmans, B.N.G. (2017). Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins. In: Clausen, B., Laman, J. (eds) Inflammation. Methods in Molecular Biology, vol 1559. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6786-5_9
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DOI: https://doi.org/10.1007/978-1-4939-6786-5_9
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Online ISBN: 978-1-4939-6786-5
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