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Inflammation pp 311–318Cite as

Oxazolone-Induced Intestinal Inflammation in Adult Zebrafish

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1559))

Abstract

Zebrafish are an excellent model for the study of intestinal immunity. The availability of several transgenic reporter fish for different innate and adaptive immune cells and the high homology in terms of gut function and morphology enables in depth analysis of the process of intestinal inflammation. Here, we describe a method to induce intestinal inflammation by intra-rectal injection of the hapten oxazolone in adult zebrafish.

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References

  1. Balla KM, Lugo-Villarino G, Spitsbergen JM, Stachura DL, Hu Y, Banuelos K, Romo-Fewell O, Aroian RV, Traver D (2010) Eosinophils in the zebrafish: prospective isolation, characterization, and eosinophilia induction by helminth determinants. Blood 116(19):3944–3954. doi:10.1182/blood-2010-03-267419

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Renshaw SA, Loynes CA, Trushell DM, Elworthy S, Ingham PW, Whyte MK (2006) A transgenic zebrafish model of neutrophilic inflammation. Blood 108(13):3976–3978. doi:10.1182/blood-2006-05-024075

    Article  CAS  PubMed  Google Scholar 

  3. Lugo-Villarino G, Balla KM, Stachura DL, Banuelos K, Werneck MB, Traver D (2010) Identification of dendritic antigen-presenting cells in the zebrafish. Proc Natl Acad Sci U S A 107(36):15850–15855. doi:10.1073/pnas.1000494107

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Ellett F, Pase L, Hayman JW, Andrianopoulos A, Lieschke GJ (2011) mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish. Blood 117(4):e49–e56. doi:10.1182/blood-2010-10-314120

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Zapata A, Diez B, Cejalvo T, Gutierrez-de Frias C, Cortes A (2006) Ontogeny of the immune system of fish. Fish Shellfish Immunol 20(2):126–136. doi:10.1016/j.fsi.2004.09.005

    Article  CAS  PubMed  Google Scholar 

  6. Lam SH, Chua HL, Gong Z, Lam TJ, Sin YM (2004) Development and maturation of the immune system in zebrafish, Danio rerio: a gene expression profiling, in situ hybridization and immunological study. Dev Comp Immunol 28(1):9–28

    Article  CAS  PubMed  Google Scholar 

  7. Hu YL, Xiang LX, Shao JZ (2010) Identification and characterization of a novel immunoglobulin Z isotype in zebrafish: implications for a distinct B cell receptor in lower vertebrates. Mol Immunol 47(4):738–746. doi:10.1016/j.molimm.2009.10.010

    Article  CAS  PubMed  Google Scholar 

  8. Hansen JD, Landis ED, Phillips RB (2005) Discovery of a unique Ig heavy-chain isotype (IgT) in rainbow trout: implications for a distinctive B cell developmental pathway in teleost fish. Proc Natl Acad Sci U S A 102(19):6919–6924. doi:10.1073/pnas.0500027102

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Zhang YA, Salinas I, Li J, Parra D, Bjork S, Xu Z, LaPatra SE, Bartholomew J, Sunyer JO (2010) IgT, a primitive immunoglobulin class specialized in mucosal immunity. Nat Immunol 11(9):827–835. doi:10.1038/ni.1913

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Brugman S, Witte M, Scholman RC, Klein MR, Boes M, Nieuwenhuis EE (2014) T lymphocyte-dependent and -independent regulation of Cxcl8 expression in zebrafish intestines. J Immunol 192(1):484–491. doi:10.4049/jimmunol.1301865

    Article  CAS  PubMed  Google Scholar 

  11. Wallace KN, Pack M (2003) Unique and conserved aspects of gut development in zebrafish. Dev Biol 255(1):12–29

    Article  CAS  PubMed  Google Scholar 

  12. Mudumana SP, Wan H, Singh M, Korzh V, Gong Z (2004) Expression analyses of zebrafish transferrin, ifabp, and elastaseB mRNAs as differentiation markers for the three major endodermal organs: liver, intestine, and exocrine pancreas. Dev Dyn 230(1):165–173. doi:10.1002/dvdy.20032

    Article  CAS  PubMed  Google Scholar 

  13. Oehlers SH, Flores MV, Hall CJ, O’Toole R, Swift S, Crosier KE, Crosier PS (2010) Expression of zebrafish cxcl8 (interleukin-8) and its receptors during development and in response to immune stimulation. Dev Comp Immunol 34(3):352–359. doi:10.1016/j.dci.2009.11.007

    Article  CAS  PubMed  Google Scholar 

  14. Oehlers SH, Flores MV, Chen T, Hall CJ, Crosier KE, Crosier PS (2011) Topographical distribution of antimicrobial genes in the zebrafish intestine. Dev Comp Immunol 35(3):385–391. doi:10.1016/j.dci.2010.11.008

    Article  CAS  PubMed  Google Scholar 

  15. Gebert A, Jepson MA (1996) Is the epithelial origin of M cells controversial? Gastroenterology 111(4):1163

    Article  CAS  PubMed  Google Scholar 

  16. Wallace KN, Akhter S, Smith EM, Lorent K, Pack M (2005) Intestinal growth and differentiation in zebrafish. Mech Dev 122(2):157–173. doi:10.1016/j.mod.2004.10.009

    Article  CAS  PubMed  Google Scholar 

  17. Oehlers SH, Flores MV, Hall CJ, Okuda KS, Sison JO, Crosier KE, Crosier PS (2013) Chemically induced intestinal damage models in zebrafish larvae. Zebrafish 10(2):184–193. doi:10.1089/zeb.2012.0824

    Article  CAS  PubMed  Google Scholar 

  18. Geiger BM, Gras-Miralles B, Ziogas DC, Karagiannis AK, Zhen A, Fraenkel P, Kokkotou E (2013) Intestinal upregulation of melanin-concentrating hormone in TNBS-induced enterocolitis in adult zebrafish. PLoS One 8(12):e83194. doi:10.1371/journal.pone.0083194

    Article  PubMed  PubMed Central  Google Scholar 

  19. Goldsmith JR, Cocchiaro JL, Rawls JF, Jobin C (2013) Glafenine-induced intestinal injury in zebrafish is ameliorated by mu-opioid signaling via enhancement of Atf6-dependent cellular stress responses. Dis Model Mech 6(1):146–159. doi:10.1242/dmm.009852

    Article  CAS  PubMed  Google Scholar 

  20. Brugman S (2016) The zebrafish as a model to study intestinal inflammation. Dev Comp Immunol. doi:10.1016/j.dci.2016.02.020

    PubMed  Google Scholar 

  21. Brugman S, Liu KY, Lindenbergh-Kortleve D, Samsom JN, Furuta GT, Renshaw SA, Willemsen R, Nieuwenhuis EE (2009) Oxazolone-induced enterocolitis in zebrafish depends on the composition of the intestinal microbiota. Gastroenterology 137(5):1757–1767, e1751. doi:10.1053/j.gastro.2009.07.069

    Article  CAS  PubMed  Google Scholar 

  22. Westerfield M (2007) The zebrafish book, 5th edn. A guide for the laboratory use of zebrafish (Danio rerio). Eugene: University of Oregon Press. Paperback (4th edition available online: http://zfin.org/zf_info/zfbook)

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Acknowledgments

The authors would like to thank the Wilhelmina Children’s Hospital Utrecht (WKZ research fund) and the Sophia Children’s Hospital Research Fund (Vrienden van Sophia) Rotterdam for generously supporting this work.

Author information

Authors and Affiliations

  1. Animal Sciences Group, Cell Biology and Immunology, Wageningen University, De Elst 1, 6708, WD, Wageningen, The Netherlands

    Sylvia Brugman Ph.D.

  2. Department of Pediatrics, Wilhelmina Children’s Hospital Utrecht, University Medical Center Utrecht, Utrecht, The Netherlands

    Edward E. S. Nieuwenhuis

Authors
  1. Sylvia Brugman Ph.D.
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  2. Edward E. S. Nieuwenhuis
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Corresponding author

Correspondence to Sylvia Brugman Ph.D. .

Editor information

Editors and Affiliations

  1. Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany

    Björn E. Clausen

  2. Section Medical Physiology Department of Neuroscience, University of Groningen University Medical Center Groningen, Groningen, The Netherlands

    Jon D. Laman

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Brugman, S., Nieuwenhuis, E.E.S. (2017). Oxazolone-Induced Intestinal Inflammation in Adult Zebrafish. In: Clausen, B., Laman, J. (eds) Inflammation. Methods in Molecular Biology, vol 1559. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6786-5_21

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  • DOI: https://doi.org/10.1007/978-1-4939-6786-5_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6784-1

  • Online ISBN: 978-1-4939-6786-5

  • eBook Packages: Springer Protocols

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