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Isolation and Identification of Intestinal Myeloid Cells

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Inflammation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1559))

Abstract

The identification of conventional dendritic cells (cDCs) and macrophages (mϕ) in the intestinal mucosa has been hampered by the difficulties associated with isolating cells from the intestine and by the fact that overlapping markers have made it complicated to discriminate them accurately from each other and from other intestinal myeloid cells. Here we detail the protocols we have developed to isolate live leukocytes from steady state mouse small and large intestines and describe reliable strategies which can be used to identify bona fide cDCs, monocytes and macrophages in such preparations.

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Author information

Authors and Affiliations

  1. Centre for Immunobiology, Institute of Infection, Immunity and Inflammation, College of Veterinary, Medical and Life Sciences, University of Glasgow, Scotland, UK

    Charlotte L. Scott, Calum C. Bain & Allan McI Mowat

  2. Inflammation Research Centre (IRC), Laboratory of Immunoregulation, VIB Ghent University, Ghent (Zwijnaarde), 9052, Belgium

    Charlotte L. Scott

  3. MRC Centre for Inflammation Research, Queens Medical Research Institute, University of Edinburgh, Edinburgh, EH16 4TJ, Scotland, UK

    Calum C. Bain

Authors
  1. Charlotte L. Scott
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  2. Calum C. Bain
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  3. Allan McI Mowat
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Corresponding author

Correspondence to Allan McI Mowat .

Editor information

Editors and Affiliations

  1. Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany

    Björn E. Clausen

  2. Section Medical Physiology Department of Neuroscience, University of Groningen University Medical Center Groningen, Groningen, The Netherlands

    Jon D. Laman

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Scott, C.L., Bain, C.C., Mowat, A.M. (2017). Isolation and Identification of Intestinal Myeloid Cells. In: Clausen, B., Laman, J. (eds) Inflammation. Methods in Molecular Biology, vol 1559. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6786-5_15

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  • DOI: https://doi.org/10.1007/978-1-4939-6786-5_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6784-1

  • Online ISBN: 978-1-4939-6786-5

  • eBook Packages: Springer Protocols

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