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Fibro/Adipogenic Progenitors (FAPs): Isolation by FACS and Culture

  • Marcela Low
  • Christine Eisner
  • Fabio RossiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1556)

Abstract

Fibro/adipogenic progenitors (FAPs ) are tissue-resident mesenchymal stromal cells (MSCs). Current literature supports a role for these cells in the homeostasis and repair of multiple tissues suggesting that FAPs may have extensive therapeutic potential in the treatment of numerous diseases. In this context, it is crucial to establish efficient and reproducible procedures to purify FAP populations from various tissues. Here, we describe a protocol for the isolation and cell culture of FAPs from murine skeletal muscle using fluorescence-activated cell sorting (FACS), which is particularly useful for experiments where high cell purity is an essential requirement. Identification, isolation, and cell culture of FAPs represent powerful tools that will help us to understand the role of these cells in different conditions and facilitate the development of safe and effective new treatments for diseases.

Key words

Fibro/adipogenic progenitors FACS Cell culture Skeletal muscle Mesenchymal stromal cell 

Notes

Acknowledgments

We thank Claudia Hopkins for her collaboration and figure construction. This work was funded by CIHR MOP-97856. M.L. was supported by a fellowship from the Chilean government, and C.E. was supported by a Four-Year Doctoral Fellowship (4YF) by the University of British Columbia.

Conflict of Interest: The authors declare no conflict of interest.

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Copyright information

© Springer Science+Business Media LLC 2017

Open Access This chapter is distributed under the terms of the Creative Commons Attribution Noncommercial License, which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

Authors and Affiliations

  1. 1.Biomedical Research CentreUniversity of British ColumbiaVancouverCanada
  2. 2.Faculty of MedicineUniversity of British ColumbiaVancouverCanada

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