Fibro/Adipogenic Progenitors (FAPs): Isolation by FACS and Culture

  • Marcela Low
  • Christine Eisner
  • Fabio RossiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1556)


Fibro/adipogenic progenitors (FAPs ) are tissue-resident mesenchymal stromal cells (MSCs). Current literature supports a role for these cells in the homeostasis and repair of multiple tissues suggesting that FAPs may have extensive therapeutic potential in the treatment of numerous diseases. In this context, it is crucial to establish efficient and reproducible procedures to purify FAP populations from various tissues. Here, we describe a protocol for the isolation and cell culture of FAPs from murine skeletal muscle using fluorescence-activated cell sorting (FACS), which is particularly useful for experiments where high cell purity is an essential requirement. Identification, isolation, and cell culture of FAPs represent powerful tools that will help us to understand the role of these cells in different conditions and facilitate the development of safe and effective new treatments for diseases.

Key words

Fibro/adipogenic progenitors FACS Cell culture Skeletal muscle Mesenchymal stromal cell 



We thank Claudia Hopkins for her collaboration and figure construction. This work was funded by CIHR MOP-97856. M.L. was supported by a fellowship from the Chilean government, and C.E. was supported by a Four-Year Doctoral Fellowship (4YF) by the University of British Columbia.

Conflict of Interest: The authors declare no conflict of interest.


  1. 1.
    da Silva Meirelles L, Chagastelles PC, Nardi NB (2006) Mesenchymal stem cells reside in virtually all post-natal organs and tissues. J Cell Sci 119:2204–2213CrossRefPubMedGoogle Scholar
  2. 2.
    Joe AWB, Yi L, Natarajan A et al (2010) Muscle injury activates resident fibro/adipogenic progenitors that facilitate myogenesis. Nat Cell Biol 12:153–163CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Uezumi A, Ito T, Morikawa D et al (2011) Fibrosis and adipogenesis originate from a common mesenchymal progenitor in skeletal muscle. J Cell Sci 124:3654–3664CrossRefPubMedGoogle Scholar
  4. 4.
    Wosczyna MN, Biswas AA, Cogswell CA et al (2012) Multipotent progenitors resident in the skeletal muscle interstitium exhibit robust BMP-dependent osteogenic activity and mediate heterotopic ossification. J Bone Miner Res 27:1004–1017CrossRefPubMedPubMedCentralGoogle Scholar
  5. 5.
    Lemos DR, Babaeijandaghi F, Low M et al (2015) Nilotinib reduces muscle fibrosis in chronic muscle injury by promoting TNF-mediated apoptosis of fibro/adipogenic progenitors. Nat Med 21:786–794CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media LLC 2017

Open Access This chapter is distributed under the terms of the Creative Commons Attribution Noncommercial License, which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

Authors and Affiliations

  1. 1.Biomedical Research CentreUniversity of British ColumbiaVancouverCanada
  2. 2.Faculty of MedicineUniversity of British ColumbiaVancouverCanada

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