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Using Transgenic Zebrafish to Study Muscle Stem/Progenitor Cells

  • Phong D. Nguyen
  • Peter D. CurrieEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1556)

Abstract

Understanding muscle stem cell behaviors can potentially provide insights into how these cells act and respond during normal growth and diseased contexts. The zebrafish is an ideal model organism to examine these behaviors in vivo where it would normally be technically challenging in other mammalian models. This chapter will describe the procedures required to successfully conduct live imaging of zebrafish transgenics that has specifically been adapted for skeletal muscle.

Key words

Zebrafish Skeletal muscle Muscle progenitor Satellite cell Live imaging Timelapse Transgenics Stem cell 

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Copyright information

© Springer Science+Business Media LLC 2017

Open Access This chapter is distributed under the terms of the Creative Commons Attribution Noncommercial License, which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

Authors and Affiliations

  1. 1.Australian Regenerative Medicine InstituteMonash UniversityClaytonAustralia

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