Skip to main content

Using Transgenic Zebrafish to Study Muscle Stem/Progenitor Cells

  • Protocol
  • First Online:
Muscle Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1556))

Abstract

Understanding muscle stem cell behaviors can potentially provide insights into how these cells act and respond during normal growth and diseased contexts. The zebrafish is an ideal model organism to examine these behaviors in vivo where it would normally be technically challenging in other mammalian models. This chapter will describe the procedures required to successfully conduct live imaging of zebrafish transgenics that has specifically been adapted for skeletal muscle.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Institutional subscriptions

References

  1. Mauro A (1961) Satellite cell of skeletal muscle fibers. J Biophys Biochem Cytol 9:493–495

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Montarras D, L’Honoré A, Buckingham M (2013) Lying low but ready for action: the quiescent muscle satellite cell. FEBS J 280(17):4036–4050. doi:10.1111/febs.12372

    Article  CAS  PubMed  Google Scholar 

  3. Seger C, Hargrave M, Wang X, Chai RJ, Elworthy S, Ingham PW (2011) Analysis of Pax7 expressing myogenic cells in zebrafish muscle development, injury, and models of disease. Dev Dyn 240(11):2440–2451

    Article  CAS  PubMed  Google Scholar 

  4. Hollway GE, Bryson-Richardson RJ, Berger S, Cole NJ, Hall TE, Currie PD (2007) Whole-somite rotation generates muscle progenitor cell compartments in the developing zebrafish embryo. Dev Cell 12(2):207–219

    Article  CAS  PubMed  Google Scholar 

  5. Siegel AL, Gurevich DB, Currie PD (2013) A myogenic precursor cell that could contribute to regeneration in zebrafish and its similarity to the satellite cell. FEBS J 280(17):4074–4088. doi:10.1111/febs.12300

    Article  CAS  PubMed  Google Scholar 

  6. Chen Y-H, Wang Y-H, Chang M-Y, Lin C-Y, Weng C-W, Westerfield M, Tsai H-J (2007) Multiple upstream modules regulate zebrafish myf5 expression. BMC Dev Biol 7(1):1

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Cole NJ, Hall TE, Don EK, Berger S, Boisvert CA, Neyt C, Ericsson R, Joss J, Gurevich DB, Currie PD (2011) Development and evolution of the muscles of the pelvic fin. PLoS Biol 9(10):e1001168. doi:10.1371/journal.pbio.1001168

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Berger J, Currie PD (2013) 503unc, a small and muscle-specific zebrafish promoter. Genesis 51(6):443–447. doi:10.1002/dvg.22385

    Article  CAS  PubMed  Google Scholar 

  9. Elworthy S, Hargrave M, Knight R, Mebus K, Ingham PW (2008) Expression of multiple slow myosin heavy chain genes reveals a diversity of zebrafish slow twitch muscle fibres with differing requirements for Hedgehog and Prdm1 activity. Development 135(12):2115–2126

    Article  CAS  PubMed  Google Scholar 

  10. Nord H, Burguiere AC, Muck J, Nord C, Ahlgren U, von Hofsten J (2014) Differential regulation of myosin heavy chains defines new muscle domains in zebrafish. Mol Biol Cell 25(8):1384–1395. doi:10.1091/mbc.E13-08-0486

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. van Impel A, Zhao Z, Hermkens DMA, Roukens MG, Fischer JC, Peterson-Maduro J, Duckers H, Ober EA, Ingham PW, Schulte-Merker S (2014) Divergence of zebrafish and mouse lymphatic cell fate specification pathways. Development 141(6):1228–1238. doi:10.1242/dev.105031

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  12. Masselink W, Wong JC, Liu B, Fu J, Currie PD (2014) Low-cost silicone imaging casts for zebrafish embryos and larvae. Zebrafish 11(1):26–31. doi:10.1089/zeb.2013.0897

    Article  PubMed  Google Scholar 

  13. Bryson-Richardson RJ, Currie PD (2008) The genetics of vertebrate myogenesis. Nat Rev Genet 9(8):632–646

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Peter D. Currie .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer Science+Business Media LLC

About this protocol

Cite this protocol

Nguyen, P.D., Currie, P.D. (2017). Using Transgenic Zebrafish to Study Muscle Stem/Progenitor Cells. In: Perdiguero, E., Cornelison, D. (eds) Muscle Stem Cells. Methods in Molecular Biology, vol 1556. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-6771-1_6

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-6771-1_6

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-6769-8

  • Online ISBN: 978-1-4939-6771-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics