Characterization of Drosophila Muscle Stem Cell-Like Adult Muscle Precursors
Uncovering how muscle stem cells behave in quiescent and activated states is central to understand the basic rules governing normal muscle development and regeneration in pathological conditions. Specification of mesodermal lineages including muscle stemlike adult muscle precursors (AMPs) has been extensively studied in Drosophila providing an attractive framework for investigating muscle stem cell properties. Restricted number of AMP cells, relative ease in following their behavior, and large number of genetic tools available make fruit fly an attractive model system for studying muscle stem cells. In this chapter, we describe the recently developed tools to visualize and target the body wall and imaginal AMPs.
Key wordsDrosophila Adult muscle precursors (AMPs) Muscle stem cell Muscle development Imaging
We wish to thank all members of the Jagla lab for stimulating discussions. We are grateful to former Jagla lab members, N. Figeac, for initial characterization of AMP markers and to R. Aradhya for generating M6-GAL4 and M6-gapGFP lines. This work is supported by the ANR grant ID-CELL-SPE to KJ, the “Equipe” FRM grant to K.J., and the AFM-Téléthon grant to G.L.
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