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Characterization of Drosophila Muscle Stem Cell-Like Adult Muscle Precursors

  • Guillaume Lavergne
  • Cedric Soler
  • Monika Zmojdzian
  • Krzysztof JaglaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1556)

Abstract

Uncovering how muscle stem cells behave in quiescent and activated states is central to understand the basic rules governing normal muscle development and regeneration in pathological conditions. Specification of mesodermal lineages including muscle stemlike adult muscle precursors (AMPs) has been extensively studied in Drosophila providing an attractive framework for investigating muscle stem cell properties. Restricted number of AMP cells, relative ease in following their behavior, and large number of genetic tools available make fruit fly an attractive model system for studying muscle stem cells. In this chapter, we describe the recently developed tools to visualize and target the body wall and imaginal AMPs.

Key words

Drosophila Adult muscle precursors (AMPs) Muscle stem cell Muscle development Imaging 

Notes

Acknowledgments

We wish to thank all members of the Jagla lab for stimulating discussions. We are grateful to former Jagla lab members, N. Figeac, for initial characterization of AMP markers and to R. Aradhya for generating M6-GAL4 and M6-gapGFP lines. This work is supported by the ANR grant ID-CELL-SPE to KJ, the “Equipe” FRM grant to K.J., and the AFM-Téléthon grant to G.L.

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Copyright information

© Springer Science+Business Media LLC 2017

Open Access This chapter is distributed under the terms of the Creative Commons Attribution Noncommercial License, which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

Authors and Affiliations

  • Guillaume Lavergne
    • 1
  • Cedric Soler
    • 1
  • Monika Zmojdzian
    • 1
  • Krzysztof Jagla
    • 1
    Email author
  1. 1.GReD (Genetics, Reproduction and Development laboratory), INSERM 1103, CNRS 6293University of Clermont AuvergneClermont-FerrandFrance

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