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Simultaneous Measurement of Mitochondrial and Glycolytic Activity in Quiescent Muscle Stem Cells

  • James G. RyallEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1556)

Abstract

Cellular metabolism has recently been identified as an important regulator of cell identity, with several adult stem cell populations having been observed to undergo a shift in metabolism during important changes in cell state, such as during the shift from quiescence to proliferation. In this chapter, a method to characterize the metabolism of quiescent skeletal muscle stem cells is presented. This technique will allow for the comparison of quiescent muscle stem cells isolated from two or more different mouse models.

Key words

Metabolism Satellite cells Glycolysis Oxidative phosphorylation Mitochondria 

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Copyright information

© Springer Science+Business Media LLC 2017

Open Access This chapter is distributed under the terms of the Creative Commons Attribution Noncommercial License, which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

Authors and Affiliations

  1. 1.Stem Cell Metabolism & Regenerative Medicine Group, Basic & Clinical Myology Laboratory, Department of PhysiologyThe University of MelbourneParkvilleAustralia

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