Engraftment of FACS Isolated Muscle Stem Cells into Injured Skeletal Muscle
Skeletal muscle stem cell (MuSC) isolation and transplantation are invaluable tools to assess their capacity for self-renewal and tissue repair. Significant technical advances in recent years have led to the optimization of these approaches, improving our ability to assess MuSC regenerative potential. Here, we describe the procedures for Fluorescent Activated Cell Sorting (FACS)-based isolation of MuSC, their intramuscular transplantation, and analysis of their engraftment into host tissues.
Key wordsFACS-based cell isolation Skeletal muscle stem cells Transplantation Engraftment Muscle regeneration
This work was supported by the US National Institutes of Health (NIH) grants R01AR064873 and P30 AR061303 to AS, and US National Institutes of Health (NIH) grant F31 AR065923-01 to MT. We thank Amy Cortez, Yoav Altman, Kenny Venegas and Buddy Charbono for technical support.
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