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Engraftment of FACS Isolated Muscle Stem Cells into Injured Skeletal Muscle

  • Matthew Tierney
  • Alessandra SaccoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1556)

Abstract

Skeletal muscle stem cell (MuSC) isolation and transplantation are invaluable tools to assess their capacity for self-renewal and tissue repair. Significant technical advances in recent years have led to the optimization of these approaches, improving our ability to assess MuSC regenerative potential. Here, we describe the procedures for Fluorescent Activated Cell Sorting (FACS)-based isolation of MuSC, their intramuscular transplantation, and analysis of their engraftment into host tissues.

Key words

FACS-based cell isolation Skeletal muscle stem cells Transplantation Engraftment Muscle regeneration 

Notes

Acknowledgments

This work was supported by the US National Institutes of Health (NIH) grants R01AR064873 and P30 AR061303 to AS, and US National Institutes of Health (NIH) grant F31 AR065923-01 to MT. We thank Amy Cortez, Yoav Altman, Kenny Venegas and Buddy Charbono for technical support.

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Copyright information

© Springer Science+Business Media LLC 2017

Open Access This chapter is distributed under the terms of the Creative Commons Attribution Noncommercial License, which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

Authors and Affiliations

  1. 1.Graduate School of Biomedical SciencesSanford-Burnham-Prebys Medical Discovery InstituteLa JollaUSA
  2. 2.Development, Aging and Regeneration Program, Sanford Children’s Health Research CenterSanford-Burnham-Prebys Medical Discovery InstituteLa JollaUSA

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