Abstract
The ability to adequately measure the phosphorylation state of a protein has major biological as well as clinical relevance. Due to its variable nature, reversible protein phosphorylations are sensitive to changes in the tissue environment. StabilizorTM T1 is a system for rapid inactivation of enzymatic activity in biological samples. Enzyme inactivation is accomplished using thermal denaturation in a rapid, homogeneous, and reproducible fashion without the need of added chemical inhibitors. Using pCREB(Ser133) as a model system, the applicability of the Stabilizor system to preserve a rapidly lost phosphorylation is shown.
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Acknowledgments
The author would like to thank Inga Hansson for skillfully carrying out IHC procedures and Charlotte Emlind-Vahlu for much appreciated advice on the manuscript.
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Borén, M. (2017). Preservation of Specific Protein Signaling States Using Heat Based Stabilizor System. In: Kalyuzhny, A. (eds) Signal Transduction Immunohistochemistry. Methods in Molecular Biology, vol 1554. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6759-9_7
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DOI: https://doi.org/10.1007/978-1-4939-6759-9_7
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6759-9
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