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Assessment of MiRNA Regulation of Endothelial Progenitor Cell Mediated Angiogenesis

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Adult Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1553))

Abstract

Organ outgrowth, embryonic development, wound healing, and many such processes require the process of angiogenesis, whereby new blood vessels are developed from the preexisting vessels. microRNAs (miRs) are 18–24 nucleotide-containing endogenous RNAs that, via a posttranscriptional mechanism, exert substantial gene regulatory effects. It was discovered by recent advances that, through direct targeting of certain critical secretory factors and transcription factors, miRs exert potent angiogenic control in a cell autonomous and non-cell autonomous manner. This chapter comprehensively summarizes step-by-step protocols for the (1) transfection of miRNA in EPCs (2) advantages and limitations of the principal tubule formation assays in use.

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Acknowledgments

This work was supported, in part, by the National Institutes of Health grants 1R01HL116729 to Dr. Krishnamurthy and American Heart Association grant-in-aid GRNT 25860041 to Dr. Krishnamurthy.

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Authors and Affiliations

  1. Department of Biomedical Engineering, University of Alabama at Birmingham, 1675 University Blvd., Volker Hall G094, Birmingham, AL, 35294, USA

    Darukeshwara Joladarashi & Prasanna Krishnamurthy

Authors
  1. Darukeshwara Joladarashi
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  2. Prasanna Krishnamurthy
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Corresponding author

Correspondence to Prasanna Krishnamurthy .

Editor information

Editors and Affiliations

  1. Center for Regenerative Medicine, Università di Roma Tor Vergata, Rome, Italy

    Paolo Di Nardo

  2. Institute of Cardiovascular Sciences St-Boniface Hospital Albrechtsen Research Centre Regenerative Medicine Program College of Medicine Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada

    Sanjiv Dhingra

  3. Division of Metabolic and Cardiovascular Sciences Burnett School of Biomedical Sciences, University of Central Florida, Orlando, Florida, USA

    Dinender K. Singla

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Joladarashi, D., Krishnamurthy, P. (2017). Assessment of MiRNA Regulation of Endothelial Progenitor Cell Mediated Angiogenesis. In: Di Nardo, P., Dhingra, S., Singla, D. (eds) Adult Stem Cells. Methods in Molecular Biology, vol 1553. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6756-8_24

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  • DOI: https://doi.org/10.1007/978-1-4939-6756-8_24

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6754-4

  • Online ISBN: 978-1-4939-6756-8

  • eBook Packages: Springer Protocols

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