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Three-Dimensional Printed Scaffolds with Multipotent Mesenchymal Stromal Cells for Rabbit Mandibular Reconstruction and Engineering

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Adult Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1553))

Abstract

Multipotent mesenchymal stromal cells (MSC) derived from both the bone marrow and adipose tissue possess the ability to differentiate into multiple cell lineages, regulate the immune function by secreting numerous bioactive paracrine factors, and hold great potential in cell therapy and tissue engineering. When combined with three-dimensional (3D) scaffolds, MSC can be used for bone defect reconstruction and engineering. This protocol describes the isolation of bone marrow mesenchymal stromal cells (BMMSC) and adipose-tissue derived stem cells (ADSC) from rabbits for subsequent seeding on tissue-engineered 3D-printed scaffolds and transplantation into a rabbit-model with the goal of repairing large osseous mandibular defects (one quarter of the lower jaw is removed surgically). Steps to demonstrate the three cell differentiation lineage potentials of BMMSC and ADSC into osteocytes, adipocytes, and chondrocytes are described. A modified cell seeding method using syringes on scaffold is detailed. Creating a large mandibular bone defect, the rapid prototyping method to print a customized 3D-scaffold, the scaffold implantation procedure in rabbits, and microcomputed tomography (micro-CT) analysis are also described.

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Acknowledgments

We would like to thank the animal resource center at McGill University for the housing and surgery preparation. The authors would like to thank the following funding agency: Natural Sciences and Engineering Research Council of Canada (NSERC), Canadian Institutes of Health Research (CIHR), and Fonds de recherche du Québec-Nature et technologies (FRQNT).

Author’s Contribution

This study was designed by D.F., M.R., M.N.A., F.T., S.T. D.F., J.J. conducted the isolation and characterization of MSCs. D.F., M.R., and M.B. conducted the transplantation of scaffolds. MNA contributed to the scaffold analysis and micro-CT scan. D.F., M.R., M.N.A., M.B., L-C.L., and S.T. wrote and revised the manuscript. S.T. supervised this study and directed final version of all contents. All authors reviewed and approved the manuscript.

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Authors and Affiliations

  1. Craniofacial Tissue Engineering and Stem Cells Laboratory, Faculty of Dentistry, McGill University, 3640 University Street, M43, Montreal, QC, Canada, H3A 0C7

    Dongdong Fang, Michael Roskies, Mohammed Bakkar, Jack Jordan, Li-Chieh Lin & Simon D. Tran

  2. Department of Otolaryngology – Head & Neck Surgery, McGill University, Montreal, QC, Canada

    Michael Roskies

  3. Faculty of Dentistry, McGill University, Montreal, QC, Canada

    Mohamed-Nur Abdallah & Faleh Tamimi

Authors
  1. Dongdong Fang
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  2. Michael Roskies
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  3. Mohamed-Nur Abdallah
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  4. Mohammed Bakkar
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  5. Jack Jordan
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  6. Li-Chieh Lin
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  7. Faleh Tamimi
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  8. Simon D. Tran
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Corresponding author

Correspondence to Simon D. Tran .

Editor information

Editors and Affiliations

  1. Center for Regenerative Medicine, Università di Roma Tor Vergata, Rome, Italy

    Paolo Di Nardo

  2. Institute of Cardiovascular Sciences St-Boniface Hospital Albrechtsen Research Centre Regenerative Medicine Program College of Medicine Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada

    Sanjiv Dhingra

  3. Division of Metabolic and Cardiovascular Sciences Burnett School of Biomedical Sciences, University of Central Florida, Orlando, Florida, USA

    Dinender K. Singla

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Fang, D. et al. (2017). Three-Dimensional Printed Scaffolds with Multipotent Mesenchymal Stromal Cells for Rabbit Mandibular Reconstruction and Engineering. In: Di Nardo, P., Dhingra, S., Singla, D. (eds) Adult Stem Cells. Methods in Molecular Biology, vol 1553. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6756-8_22

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  • DOI: https://doi.org/10.1007/978-1-4939-6756-8_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6754-4

  • Online ISBN: 978-1-4939-6756-8

  • eBook Packages: Springer Protocols

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