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Adult Stem Cells pp 241–248Cite as

Methods for Long-Term Storage of Murine Bone Marrow-Derived Mesenchymal Stem Cells

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1553))

Abstract

This chapter is based on a simplified method to validate the current preservation procedure of mesenchymal stem cells (MSCs). Currently, there are various media available for freezing and thus preserving the MSCs, making it hard to decide which agent will be apt for cellular requirements. The study describes the effect of two different compositions of freezing media used in regular cell culture experiments, on the morphology, proliferation, and doubling rate of MSCs. Commonly used agents for the cryopreservation of MSCs include DMSO (Dimethyl Sulfoxide) and FBS (Fetal Bovine Serum) and DMEM (Dulbecco’s Modified Eagle Medium). To ascertain that the currently used agents do not lead to major changes in the MSC morphology and proliferation, the cells are frozen using the above-mentioned agents in different groups and then their effects analyzed. Thus, the chapter helps to decide what reagents can suit the MSCs, hence minimizing the laboratory to laboratory variability of their characteristics.

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References

  1. Korbling M, Estrov Z (2003) Adult stem cells for tissue repair. A new therapeutic concept? N Engl J Med 349(6):570–582

    Article  PubMed  Google Scholar 

  2. Bianco P, Riminucci M, Gronthos S, Robey PG (2001) Bone marrow stromal stem cells: nature, biology, and potential applications. Stem Cells 19(3):180–192

    Article  CAS  PubMed  Google Scholar 

  3. Chamberlain G, Fox J, Ashton B, Middleton J (2007) Concise review: mesenchymal stem cells: their phenotype, differentiation capacity, immunological features, and potential for homing. Stem Cells 25:2739–2749

    Article  CAS  PubMed  Google Scholar 

  4. Jiang Y, Jahagirdar BN, Reinhardt RL, Schwartz RE, Keene CD, Ortiz-Gonzalez XR et al (2002) Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 418:41–49

    Article  CAS  PubMed  Google Scholar 

  5. Alviano F, Fossati V, Marchionni C, Arpinati M, Bonsi L, Franchina M et al (2007) Term Amniotic membrane is a high throughout source for multipotent mesenchymal stem cells with the ability to differentiate into endothelial cells in vitro. BMC Dev Biol 7:11. doi:10.1186/1471-213X-7-11

    Article  PubMed  PubMed Central  Google Scholar 

  6. Xiang Y, Zheng Q, Jia BB, Huang GP, Xu YL, Wang JF et al (2007) Ex vivo expansion, adipogenesis and neurogenesis of cryopreserved human bone marrow mesenchymal stem cells. Cell Biol Int 31(5):444–450

    Article  CAS  PubMed  Google Scholar 

  7. Bahadori MH (2012) Cryopreservation of rat bone marrow derived mesenchymal stem cells by two conventional and open-pulled straw vitrification methods. In: Katkov II (ed) Current frontiers in cryopreservation. InTech, Croatia

    Google Scholar 

  8. Viswanathan S, Keating A, Deans R, Hematti P, Prockop D, Stroncek DF, Stacey G, Weiss DJ, Mason C, Rao MS (2014) Soliciting strategies for developing cell-based reference materials to advance mesenchymal stromal cell research and clinical translation. Stem Cells Dev 23(11):1157–1167

    Article  PubMed  PubMed Central  Google Scholar 

  9. Wagner W, Horn P, Castoldi M, Diehlmann A, Bork S, Saffrich R, Benes V, Blake J, Pfister S, Eckstein V, Ho AD (2008) Replicative senescence of mesenchymal stem cells: a continuous and organized process. PLoS One 3(5):e221

    Article  Google Scholar 

  10. Marquez-Curtis LA, Janowska-Wieczorek A, McGann LE, Elliott JA (2015) Mesenchymal stromal cells derived from various tissues: biological,clinical and cryopreservation aspects. Cryobiology 71(2):181–197

    Article  CAS  PubMed  Google Scholar 

  11. Fujioka T, Yasuchika K, Nakamura Y, Nakasuji N, Suemori H (2004) A simple and efficient cryopreservation method for primate embryonic stem cells. Int J Dev Biol 48(10):1149–1154

    Article  PubMed  Google Scholar 

  12. Hunt CJ (2011) Cryopreservation of human stem cells for clinical application: a review. Transfus Med Hemother 38(2):107–123

    Article  PubMed  PubMed Central  Google Scholar 

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Author information

Authors and Affiliations

  1. Institute of Cardiovascular Sciences, St. Boniface Hospital Albrechtsen Research Centre, Regenerative Medicine Program, College of Medicine, Faculty of Health Sciences, University of Manitoba, Winnipeg, MB, Canada

    Niketa Sareen, Ejlal Abu-El-Rub, Glen Lester Sequiera & Meenal Moudgil

  2. Institute of Cardiovascular Sciences, St. Boniface Hospital Albrechtsen Research Centre, R 3028-2, 351 Tache Ave., Winnipeg, MB, R2H2A6, Canada

    Sanjiv Dhingra

Authors
  1. Niketa Sareen
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  2. Ejlal Abu-El-Rub
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  3. Glen Lester Sequiera
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  4. Meenal Moudgil
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  5. Sanjiv Dhingra
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Corresponding author

Correspondence to Sanjiv Dhingra .

Editor information

Editors and Affiliations

  1. Center for Regenerative Medicine, Università di Roma Tor Vergata, Rome, Italy

    Paolo Di Nardo

  2. Institute of Cardiovascular Sciences St-Boniface Hospital Albrechtsen Research Centre Regenerative Medicine Program College of Medicine Faculty of Health Sciences, University of Manitoba, Winnipeg, Manitoba, Canada

    Sanjiv Dhingra

  3. Division of Metabolic and Cardiovascular Sciences Burnett School of Biomedical Sciences, University of Central Florida, Orlando, Florida, USA

    Dinender K. Singla

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Sareen, N., Abu-El-Rub, E., Sequiera, G.L., Moudgil, M., Dhingra, S. (2017). Methods for Long-Term Storage of Murine Bone Marrow-Derived Mesenchymal Stem Cells. In: Di Nardo, P., Dhingra, S., Singla, D. (eds) Adult Stem Cells. Methods in Molecular Biology, vol 1553. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6756-8_19

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  • DOI: https://doi.org/10.1007/978-1-4939-6756-8_19

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6754-4

  • Online ISBN: 978-1-4939-6756-8

  • eBook Packages: Springer Protocols

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