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Proteomics pp 19-33 | Cite as

Proteome Characterization of a Chromatin Locus Using the Proteomics of Isolated Chromatin Segments Approach

  • Sophie L. Kan
  • Nehmé Saksouk
  • Jérome DéjardinEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1550)

Abstract

The biological functions of given genomic regions are ruled by the local chromatin composition. The Proteomics of Isolated Chromatin segments approach (PICh) is a powerful and unbiased method to analyze the composition of chosen chromatin segments, provided they are abundant (repeated) or that the organism studied has a small genome. PICh can be used to identify novel and unexpected regulatory factors, or when combined with quantitative mass spectrometric approaches, to characterize the function of a defined factor at the chosen locus, by quantifying composition changes at the locus upon removal/addition of that factor.

Key words

Proteomics Chromatin Protein Purification Repeated Sequences Heterochromatin Telomeres 

Notes

Acknowledgments

We would like to thank Titia de Lange for the kind gift of the TRF1 antibody.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Sophie L. Kan
    • 1
  • Nehmé Saksouk
    • 1
  • Jérome Déjardin
    • 1
    Email author
  1. 1.INSERM AVENIR, Institute of Human Genetics CNRS UPR1142MontpellierFrance

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