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Proteomics pp 271-288 | Cite as

Designing Successful Proteomics Experiments

  • Daniel RudermanEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1550)

Abstract

Because proteomics experiments are so complex they can readily fail, and do so without clear cause. Using standard experimental design techniques and incorporating quality control can greatly increase the chances of success. This chapter introduces the relevant concepts and provides examples specific to proteomic workflows. Applying these notions to design successful proteomics experiments is straightforward. It can help identify failure causes and greatly increase the likelihood of inter-laboratory reproducibility.

Key words

Design of experiments Randomization Bias Variance 

Notes

Acknowledgments

I thank Dr. Parag Mallick for introducing me to the field of experimental design and Dr. Nicholas Graham for comments on the manuscript.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Lawrence J. Ellison Institute for Transformative Medicine of USCKeck School of Medicine of USCLos AngelesUSA

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