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Proteomics pp 235-260 | Cite as

Annotating Mutational Effects on Proteins and Protein Interactions: Designing Novel and Revisiting Existing Protocols

  • Minghui Li
  • Alexander Goncearenco
  • Anna R. PanchenkoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1550)

Abstract

In this review we describe a protocol to annotate the effects of missense mutations on proteins, their functions, stability, and binding. For this purpose we present a collection of the most comprehensive databases which store different types of sequencing data on missense mutations, we discuss their relationships, possible intersections, and unique features. Next, we suggest an annotation workflow using the state-of-the art methods and highlight their usability, advantages, and limitations for different cases. Finally, we address a particularly difficult problem of deciphering the molecular mechanisms of mutations on proteins and protein complexes to understand the origins and mechanisms of diseases.

Key words

Protein–protein interactions Databases Mutations 

Notes

Acknowledgements

This work was supported by the Intramural Research Program of the National Library of Medicine.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Minghui Li
    • 1
  • Alexander Goncearenco
    • 1
  • Anna R. Panchenko
    • 1
    Email author
  1. 1.National Center for Biotechnology InformationNational Institutes of HealthBethesdaUSA

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