Proteomics pp 171-184 | Cite as

Quantitative Proteomics Using SILAC

  • Kian KaniEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1550)


The ability to enumerate all of the proteins in a cell is quickly becoming a reality. Quantitative proteomics adds an extra dimension to proteome-wide discovery experiments by enabling differential measurements of protein concentrations, characterization of protein turnover, increased stringency of co-immunoprecipitation reactions, as well as many other intriguing applications. One of the most widely used techniques that enable relative protein quantitation is stable isotope labeling by amino acids in cell culture (SILAC) (Ong et al., Mol Cell Proteomics 1(5):376–386, 2002). Over the past decade, SILAC has become the preferred approach for proteome-wide quantitation by mass spectrometry. This approach relies on the metabolic incorporation of isotopically enriched amino acids into the proteome of cells—the proteome of “light” (1H, 12C, 14N) cells can then be compared to “heavy” (2H, 13C, 15N) cells as the isotopically labeled proteins and peptides are easily distinguished in a mass spectrometer. Since cellular uptake and response to isotopically different amino acid(s) is naïve, it is without impact on cell physiology. We provide a detailed step-by-step procedure for performing SILAC-based experiment for proteome-wide quantitation in this chapter.

Key words

SILAC AMT Quantitative Proteomics Multiplex 


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.USC Center for Applied Molecular MedicineUSC Keck School of MedicineLos AngelesUSA

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