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Analyzing Targeted Nucleosome Position and Occupancy in Cancer, Obesity, and Diabetes

  • Prasad P. Devarshi
  • Tara M. HenaganEmail author
Protocol
  • 806 Downloads
Part of the Methods in Pharmacology and Toxicology book series (MIPT)

Abstract

Chromatin structure plays an integral role in regulation of gene transcription. Studying nucleosome position and occupancy at key regulatory regions within DNA is important to understanding the potential epigenetic mechanisms of gene regulation during various environmental exposures and diseased states. Targeted nucleosome mapping is a convenient method to map nucleosome positions at a specific genomic locus. In this method, mononucleosomal DNA is isolated using micrococcal nuclease. This is followed by qPCR, which uses the mononucleosomal DNA, or the DNA bound by the nucleosome, as the template and overlapping primers that span the target region of genome. qPCR products are analyzed by gel electrophoresis and densitometry to yield a map of nucleosomes along specific, targeted genomic loci.

Key words

Nucleosome Epigenetics Scanning PCR Cancer Obesity Type 2 diabetes 

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  1. 1.Department of Nutrition SciencePurdue UniversityWest LafayetteUSA

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