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High-Performance Liquid Chromatography and Mass Spectrometry-Based Design of Proteolytically Stable Antimicrobial Peptides

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Antimicrobial Peptides

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1548))

Abstract

The emergence of multiresistant bacteria worldwide together with the shortage of effective antibiotics in the market emphasizes the need for the design and development of the promising agents for the treatment of superbug-associated infections. Antimicrobial peptides (AMPs) have been considered as excellent candidates to tackle this issue, and thousands of peptides of different lengths, amino acid compositions, and mode of action have been discovered and prepared to date. Nevertheless, it is of great importance to develop innovative formulation strategies for delivering these AMPs and to improve their low bioavailability and metabolic stability, particularly against proteases, if these peptides are to find applications in the clinic and administered orally or parenterally or used as dietary supplements. The purpose of this chapter is to describe basic experimental principles, based on analytical reversed-phase high-performance liquid chromatography (RP-HPLC) and mass spectrometry (MS), for the prospective design of orally bioavailable AMPs considering the structural characteristics of the peptides and the substrate specificity of proteases that abound in the body especially at sites of infection.

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Acknowledgments

MB would thank the Iran National Science Foundation (reference No. 94012757), Iran’s National Elites Foundation (reference No. 15/45362–1392), and University of Tehran for the financial supports. REWH would like to acknowledge research funding from the Canadian Institutes for Health Research, and he is the holder of a Canada Research Chair in Health and Genomics.

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Authors and Affiliations

  1. Peptide Chemistry Laboratory, Institute of Biochemistry and Biophysics, University of Tehran, 16 Azar Street, 1417614335, Tehran, Iran

    Mojtaba Bagheri

  2. Department of Microbiology and Immunology, Center forMicrobial Diseases and Immunity Research, University of British Columbia, Vancouver, BC, Canada

    Robert E. W. Hancock

Authors
  1. Mojtaba Bagheri
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  2. Robert E. W. Hancock
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Correspondence to Mojtaba Bagheri .

Editor information

Editors and Affiliations

  1. Department of Drug Design and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

    Paul R. Hansen

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Bagheri, M., Hancock, R.E.W. (2017). High-Performance Liquid Chromatography and Mass Spectrometry-Based Design of Proteolytically Stable Antimicrobial Peptides. In: Hansen, P. (eds) Antimicrobial Peptides. Methods in Molecular Biology, vol 1548. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6737-7_5

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  • DOI: https://doi.org/10.1007/978-1-4939-6737-7_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6735-3

  • Online ISBN: 978-1-4939-6737-7

  • eBook Packages: Springer Protocols

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