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Droplet Microfluidic and Magnetic Particles Platform for Cancer Typing

  • Davide Ferraro
  • Jérôme Champ
  • Bruno Teste
  • M. Serra
  • Laurent Malaquin
  • Stéphanie Descroix
  • Patricia de Cremoux
  • Jean-Louis ViovyEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1547)

Abstract

Analyses of nucleic acids are routinely performed in hospital laboratories to detect gene alterations for cancer diagnosis and treatment decision. Among the different possible investigations, mRNA analysis provides information on abnormal levels of genes expression. Standard laboratory methods are still not adapted to the isolation and quantitation of low mRNA amounts and new techniques needs to be developed in particular for rare subsets analysis. By reducing the volume involved, time process, and the contamination risks, droplet microfluidics provide numerous advantages to perform analysis down to the single cell level.

We report on a droplet microfluidic platform based on the manipulation of magnetic particles that allows the clinical analysis of tumor tissues. In particular, it allows the extraction of mRNA from the total-RNA sample, Reverse Transcription, and cDNA amplification, all in droplets.

Key words

Droplet microfluidics Magnetic tweezers Reverse Transcription (RT) PCR 

Notes

Acknowledgments

This work was supported by the NADINE FP7 project, ANR DigiDiag (ANR 10-NANO-02-03) and by the Foundation ARC (young researcher fellowship D.F.). Some technological elements used in this article are covered by patent WO2013041983, property of Centre National de la Recherche Scientifique.

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Davide Ferraro
    • 1
  • Jérôme Champ
    • 1
    • 2
    • 3
  • Bruno Teste
    • 1
  • M. Serra
    • 1
  • Laurent Malaquin
    • 1
  • Stéphanie Descroix
    • 1
  • Patricia de Cremoux
    • 2
    • 3
  • Jean-Louis Viovy
    • 1
    Email author
  1. 1.Macromolecules and Microsystems in Biology and MedicineInstitut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research UniversityParisFrance
  2. 2.University Paris-Diderot, PRES Paris Cité, INSERM/CNRS UMR944/7212ParisFrance
  3. 3.Molecular Oncology UnitAPHP, Saint-Louis HospitalParisFrance

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