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Digital Microfluidics Assisted Sealing of Individual Magnetic Particles in Femtoliter-Sized Reaction Wells for Single-Molecule Detection

  • Deborah Decrop
  • Elena Pérez Ruiz
  • Phalguni Tewari Kumar
  • Lisa Tripodi
  • Tadej Kokalj
  • Jeroen LammertynEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1547)

Abstract

Digital microfluidics has emerged in the last years as a promising liquid handling technology for a variety of applications. Here, we describe in detail how to build up an electrowetting-on-dielectric-based digital microfluidic chip with unique advantages for performing single-molecule detection. We illustrate how superparamagnetic particles can be printed with very high loading efficiency (over 98 %) and single-particle resolution in the microwell array patterned in the Teflon-AF® surface of the grounding plate of the chip. Finally, the potential of the device for its application to single-molecule detection is demonstrated by the ultrasensitive detection of the biotinylated enzyme β-Galactosidase captured on streptavidin-coated particles in the described platform.

Key words

Digital Microfluidics Microwell Array Magnetic Particles Single-Molecule Detection Digital Bioassays 

Notes

Acknowledgments

This research was financially supported by the KU Leuven Research Council (IDO-project 10/012, OT project 13/058 and Atheromix IOF-knowledge platform), the Agency for Innovation by Science and Technology in Flanders (IWT project 121615), and the Fund for Scientific Research Flanders—FWO (G.0997.11 and G.0861.14).

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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Deborah Decrop
    • 1
  • Elena Pérez Ruiz
    • 1
  • Phalguni Tewari Kumar
    • 1
  • Lisa Tripodi
    • 1
  • Tadej Kokalj
    • 1
  • Jeroen Lammertyn
    • 1
    Email author
  1. 1.MeBioS - Biosensors, Department of BiosystemsKU LeuvenLeuvenBelgium

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