Using Electrophoretic Immunoassay to Monitor Hormone Secretion
It has been demonstrated that microfluidic systems allow integration of sampling, reagent mixing, and rapid electrophoretic analysis. They have also proven useful for culturing cells wherein control over the environment allows novel and automated experiments. Here, we describe a microchip-based electrophoresis assay that allows cell culture and hormone monitoring. An online gradient generator can control cell culture condition precisely. This system has been applied for Pancreas islets’ glucose sensitivity studies.
Key wordsMicrofluidics Perfusion culture Capillary electrophoresis Competitive immunoassay Islets of Langerhans Diabetes Glucose sensitivity
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