Fabrication and Evaluation of Microfluidic Immunoassay Devices with Antibody-Immobilized Microbeads Retained in Porous Hydrogel Micropillars

  • Toshihiro Kasama
  • Noritada Kaji
  • Manabu Tokeshi
  • Yoshinobu BabaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1547)


Due to the inherent characteristics including confinement of molecular diffusion and high surface-to-volume ratio, microfluidic device-based immunoassay has great advantages in cost, speed, sensitivity, and so on, compared with conventional techniques such as microtiter plate-based ELISA, latex agglutination method, and lateral flow immunochromatography. In this paper, we explain the detection of C-reactive protein as a model antigen by using our microfluidic immunoassay device, so-called immuno-pillar device. We describe in detail how we fabricated and used the immuno-pillar devices.

Key words

Lab-on-a-chip Microfluidic immunoassay Biomarker 



This work was partially supported by the Japan Society for the Promotion of Science (JSPS, grant no. 24245013). We would like to thank Kansai Paint Co., Ltd., for valuable discussions.


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Copyright information

© Springer Science+Business Media LLC 2017

Authors and Affiliations

  • Toshihiro Kasama
    • 1
  • Noritada Kaji
    • 1
    • 2
  • Manabu Tokeshi
    • 1
    • 3
  • Yoshinobu Baba
    • 1
    • 3
    • 4
    • 5
    • 6
    Email author
  1. 1.Department of Applied Chemistry, Graduate School of EngineeringNagoya UniversityNagoyaJapan
  2. 2.ImPACT Research Center for Advanced NanobiodevicesNagoya University, Furo-cho, Chikusa-kuNagoyaJapan
  3. 3.Division of Biotechnology and Macromolecular ChemistryHokkaido UniversitySapporoJapan
  4. 4.Institute of Innovation for Future SocietyNagoya UniversityNagoyaJapan
  5. 5.Health Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)TakamatsuJapan
  6. 6.ImPACT Research Center for Advanced NanobiodevicesNagoya University, Furo-cho, Chikusa-kuNagoyaJapan

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