Abstract
The enumeration and analysis of circulating tumor cells (CTCs) is an increasing interest for monitoring disease progression or response to treatment, specifically as a companion diagnostic for new anticancer drugs, and for research into the mechanisms of disease progression and metastases. Ideally, CTCs would be enriched from very small samples, with minimal handling, high recovery, and no requirement for the expression of specific surface markers. Here, we describe negative enrichment as the preferred approach for cancer cell isolation using a microfluidic platform.
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Acknowledgments
This work was supported by the Science and Engineering Research Council of A*STAR (Agency for Science, Technology and Research), Singapore under the grant number 1031490005.
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Cognart, H.A., Chang, CP. (2017). Negative Enrichment of Circulating Tumor Cells in Blood Using a Microfluidic Chip. In: Taly, V., Viovy, JL., Descroix, S. (eds) Microchip Diagnostics. Methods in Molecular Biology, vol 1547. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6734-6_13
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DOI: https://doi.org/10.1007/978-1-4939-6734-6_13
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6734-6
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