Abstract
Multiplex immunoassays allow for the rapid profiling of biomarker proteins in biological fluids, using less sample and labor than single immunoassays. This chapter details the methods to develop and manufacture multiplex assays for the Luminex® platform. Although assay development is not included here, the same methods can be used to covalently couple antibodies to the Luminex beads and to label antibodies for the screening of sandwich pairs, if needed. The assay optimization, detection of cross-reactivity, and minimizing antibody interactions and matrix interferences will be addressed.
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Stephen, L. (2017). Multiplex Immunoassay Profiling. In: Guest, P.C. (eds) Multiplex Biomarker Techniques. Methods in Molecular Biology, vol 1546. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-6730-8_13
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DOI: https://doi.org/10.1007/978-1-4939-6730-8_13
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-4939-6729-2
Online ISBN: 978-1-4939-6730-8
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