Abstract
Pulsed stable isotope labeling by amino acids in cell culture (pSILAC) comprises a variation of the classical SILAC proteomic methodology that enables the identification of short-term proteomic responses such as those elicited by micro RNAs (miRNAs). Here, we describe a detailed pSILAC protocol for global identification and quantification of protein translation alterations induced by a miRNA using 3T3-L1 pre-adipocytes as a model system.
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Acknowledgments
DDG was the recipient of a FAPESP Post-Doctoral Fellowship (BEPE-PD 2014/20380-5) and is funded by the BHF (PG/14/20/30769. JdAF was the recipient of a FAPESP Doctoral Fellowship (BEPE-DR 2014/17012-4),TPO was a recipient of a Visiting Scientist CAPES Science Without Borders Fellowship (BEX 11766-13-1). SEO is funded by the UK Medical Research Council (MC_UU_12012/4).
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Duque-Guimarães, D.E., de Almeida-Faria, J., Ong, T.P., Ozanne, S.E. (2017). Pulsed SILAC as a Approach for miRNA Targets Identification in Cell Culture. In: Guest, P.C. (eds) Multiplex Biomarker Techniques. Methods in Molecular Biology, vol 1546. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-6730-8_11
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DOI: https://doi.org/10.1007/978-1-4939-6730-8_11
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Publisher Name: Humana, New York, NY
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