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Mycotoxigenic Fungi pp 293–309Cite as

Multiplex Detection of Toxigenic Penicillium Species

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1542))

Abstract

Multiplex PCR-based methods for simultaneous detection and quantification of different mycotoxin-producing Penicillia are useful tools to be used in food safety programs. These rapid and sensitive techniques allow taking corrective actions during food processing or storage for avoiding accumulation of mycotoxins in them. In this chapter, three multiplex PCR-based methods to detect at least patulin- and ochratoxin A-producing Penicillia are detailed. Two of them are different multiplex real-time PCR suitable for monitoring and quantifying toxigenic Penicillium using the nonspecific dye SYBR Green and specific hydrolysis probes (TaqMan). All of them successfully use the same target genes involved in the biosynthesis of such mycotoxins for designing primers and/or probes.

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Acknowledgments

This work was supported by AGL2010-21623 and Carnisenusa CSD2007-00016—ConsoliderIngenio 2010 from the Spanish Comision Interministerial de Ciencia y Tecnología projects and GR10162 of the Junta de Extremadura and FEDER. Dr. A. Rodríguez is supported by a “Juan de la Cierva-Incorporación” Senior Research Fellowship (IJCI-2014-20666) from the Spanish Ministry of Economy and Competitiveness.

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Authors and Affiliations

  1. Faculty of Veterinary Science, Food Hygiene and Safety, Meat and Meat Products Research Institute, University of Extremadura, Avda de las Ciencias s/n, 10003, Cáceres, Spain

    Alicia Rodríguez, Juan J. Córdoba, Mar Rodríguez & María J. Andrade

Authors
  1. Alicia Rodríguez
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  2. Juan J. Córdoba
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  3. Mar Rodríguez
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  4. María J. Andrade
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Corresponding author

Correspondence to Alicia Rodríguez .

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Editors and Affiliations

  1. Institute of Sciences of Food Production National Research Council, Bari, Italy

    Antonio Moretti

  2. Institute of Sciences of Food Production National Research Council, Bari, Italy

    Antonia Susca

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Rodríguez, A., Córdoba, J.J., Rodríguez, M., Andrade, M.J. (2017). Multiplex Detection of Toxigenic Penicillium Species. In: Moretti, A., Susca, A. (eds) Mycotoxigenic Fungi. Methods in Molecular Biology, vol 1542. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6707-0_19

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  • DOI: https://doi.org/10.1007/978-1-4939-6707-0_19

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6705-6

  • Online ISBN: 978-1-4939-6707-0

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